Ion exchange chromatography column, method, and system thereof
First Claim
1. A chromatography column, the column containing a packed bed of an ion exchange chromatographic packing material, the ion exchange chromatographic packing material comprising:
- a) support resin particles; and
b) a copolymer comprising polymerized functional monomers that include a first ion exchange group monomer and a second ion exchange group monomer, where the first ion exchange group monomer and the second ion exchange group monomer are different,in which the first ion exchange group monomer is configured to have a first charge at a first pH, and the second ion exchange group monomer is configured to have a net neutral charge at the first pH,in which the first ion exchange group monomer is configured to have the first charge at a second pH, and the second ion exchange group monomer is configured to have a net neutral charge or a second charge at the second pH where the first charge and the second charge both have a same polarity, in which the first pH and the second pH are different,in which a mole percent of the second ion exchange group monomer is a mole amount of the second ion exchange group monomer divided by a summation of a mole amount of the first ion exchange group monomer and the mole amount of the second ion exchange group monomer, and multiplied by 100, the mole percent of the second ion exchange group monomer ranges from about 60% to about 99%,in which the copolymer is grafted to the support resin particles, in which the first ion exchange group monomer comprises a sulfonate group and the second ion exchange group monomer comprises a carboxylic acid group.
1 Assignment
0 Petitions
Accused Products
Abstract
An ion exchange chromatographic packing material is described that includes support resin particles and a copolymer grafted to the support resin particles. The copolymer includes polymerized functional monomers such as a first ion exchange group monomer and a second ion exchange group monomer. At a first pH, the first ion exchange group monomer is configured to have a first charge at a first pH, and the second ion exchange group monomer is configured to have a net neutral charge. At a second pH, the first ion exchange group monomer is configured to have the first charge at a second pH, and the second ion exchange group monomer is configured to have a second charge at the second pH where the first charge and second charge both have a same polarity.
17 Citations
20 Claims
-
1. A chromatography column, the column containing a packed bed of an ion exchange chromatographic packing material, the ion exchange chromatographic packing material comprising:
-
a) support resin particles; and b) a copolymer comprising polymerized functional monomers that include a first ion exchange group monomer and a second ion exchange group monomer, where the first ion exchange group monomer and the second ion exchange group monomer are different, in which the first ion exchange group monomer is configured to have a first charge at a first pH, and the second ion exchange group monomer is configured to have a net neutral charge at the first pH, in which the first ion exchange group monomer is configured to have the first charge at a second pH, and the second ion exchange group monomer is configured to have a net neutral charge or a second charge at the second pH where the first charge and the second charge both have a same polarity, in which the first pH and the second pH are different, in which a mole percent of the second ion exchange group monomer is a mole amount of the second ion exchange group monomer divided by a summation of a mole amount of the first ion exchange group monomer and the mole amount of the second ion exchange group monomer, and multiplied by 100, the mole percent of the second ion exchange group monomer ranges from about 60% to about 99%, in which the copolymer is grafted to the support resin particles, in which the first ion exchange group monomer comprises a sulfonate group and the second ion exchange group monomer comprises a carboxylic acid group. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
-
-
19. A system for chromatographically separating a sample, the system comprising:
-
a pump configured to pump a mobile phase containing the sample from a liquid source; an electrolytic eluent generating device fluidically connected to the pump and configured to generate an eluent stream; an injection valve configured to inject an aliquot of the sample into the eluent stream; a chromatography column fluidically connected to the injection valve and configured to separate one or more analytes from matrix components in the sample, the chromatography column containing a packed bed of an ion exchange chromatographic packing material, the ion exchange chromatographic packing material comprising; a) support resin particles; and b) a copolymer comprising polymerized functional monomers that include a first ion exchange group monomer and a second ion exchange group monomer, where the first ion exchange group monomer and the second ion exchange group monomer are different, in which the first ion exchange group monomer is configured to have a first charge at a first pH, and the second ion exchange group monomer is configured to have a net neutral charge at the first pH, in which the first ion exchange group monomer is configured to have the first charge at a second pH, and the second ion exchange group monomer is configured to have a net neutral charge or a second charge at the second pH where the first charge and the second charge both have a same polarity, in which the first pH and the second pH are different, in which a mole percent of the second ion exchange group monomer is a mole amount of the second ion exchange group monomer divided by a summation of a mole amount of the first ion exchange group monomer and the mole amount of the second ion exchange group monomer, and multiplied by 100, the mole percent of the second ion exchange group monomer ranges from about 60% to about 99%, in which the copolymer is grafted to the support resin particles, in which the first ion exchange group monomer comprises a sulfonate group and the second ion exchange group monomer comprises a carboxylic acid group; and a detector fluidically connected to an output of the chromatography column.
-
-
20. A method of separating a sample, the method comprising:
flowing a mobile phase containing a plug of the sample into a chromatography column, the column containing a packed bed of an ion exchange chromatographic packing material, the ion exchange chromatographic packing material comprising; a) support resin particles; and b) a copolymer comprising polymerized functional monomers that include a first ion exchange group monomer and a second ion exchange group monomer, where the first ion exchange group monomer and the second ion exchange group monomer are different, in which the first ion exchange group monomer is configured to have a first charge at a first pH, and the second ion exchange group monomer is configured to have a net neutral charge at the first pH, in which the first ion exchange group monomer is configured to have the first charge at a second pH, and the second ion exchange group monomer is configured to have a net neutral charge or a second charge at the second pH where the first charge and the second charge both have a same polarity, in which the first pH and the second pH are different, in which a mole percent of the second ion exchange group monomer is a mole amount of the second ion exchange group monomer divided by a summation of a mole amount of the first ion exchange group monomer and the mole amount of the second ion exchange group monomer, and multiplied by 100, the mole percent of the second ion exchange group monomer ranges from about 60% to about 99%, in which the copolymer is grafted to the support resin particles, in which the first ion exchange group monomer comprises a sulfonate group and the second ion exchange group monomer comprises a carboxylic acid group;
separating the sample into one or more analyte bands in the chromatography column; and
detecting the one or more analyte bands eluting off the chromatography column.
Specification