Method for rapid accurate dispensing, visualization and analysis of single cells
First Claim
1. A method comprising:
- a) dispensing with a liquid dispensing system a dispense volume of a cell suspension into each of at least 75 wells in a multi-well device so that each of the at least 75 wells contains one cell, zero cells or more than one cell and at least some of the wells contain zero cells, wherein said cell suspension comprises cells present at a concentration such that, on average, one cell is present in said dispense volume;
b) capturing a digital image of at least some of the at least 75 wells using an image capturing system;
c) generating a dispense map from the digital image and storing the dispense map to a computer memory, wherein the dispense map provides instructions to the liquid dispensing system to only dispense a reagent into wells containing only one cell; and
d) dispensing, with the liquid dispensing system, the reagent into wells containing only one cell based on the generated dispense map stored in the computer memory.
2 Assignments
0 Petitions
Accused Products
Abstract
The present disclosure provides methods, device, assemblies, and systems for dispensing and visualizing single cells. For example, provided herein are systems and methods for dispensing a dispense volume into a plurality of wells of a multi-well device, where, on average, a pre-determined number of cells (e.g., 1-20) are present in the dispense volume, and determining, via a cellular label, the number of cells present in each of the plurality of wells. Such dispensing and cell detection may be repeated a number of times with respect to wells identified as having less than the pre-determined number of cells in order increase the number wells in the multi-well device containing the desired number (e.g., a single cell).
-
Citations
26 Claims
-
1. A method comprising:
-
a) dispensing with a liquid dispensing system a dispense volume of a cell suspension into each of at least 75 wells in a multi-well device so that each of the at least 75 wells contains one cell, zero cells or more than one cell and at least some of the wells contain zero cells, wherein said cell suspension comprises cells present at a concentration such that, on average, one cell is present in said dispense volume; b) capturing a digital image of at least some of the at least 75 wells using an image capturing system; c) generating a dispense map from the digital image and storing the dispense map to a computer memory, wherein the dispense map provides instructions to the liquid dispensing system to only dispense a reagent into wells containing only one cell; and d) dispensing, with the liquid dispensing system, the reagent into wells containing only one cell based on the generated dispense map stored in the computer memory. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
-
-
22. A system comprising:
-
a) a multi-well device comprising at least 75 wells; b) a liquid dispensing system that dispenses a dispense volume of a cell suspension into each of said at least 75 wells so that each of the at least 75 wells contains one cell, zero cells or more than one cell and at least some of the wells contain zero cells; c) an image capturing system configured to capture an image of at least some of the at least 75 wells; and d) a dispense map file based on the captured image that provides instructions to said liquid dispensing system to only dispense a reagent into wells containing only one cell. - View Dependent Claims (23)
-
-
24. A system comprising:
-
a) a multi-well device comprising at least 75 wells; b) a liquid dispensing system that dispenses a dispense volume of a cell suspension into each of said at least 75 wells so that each of the at least 75 wells contains one cell, zero cells or more than one cell and at least some of the wells contain zero cells; c) an image capturing system configured to capture an image of at least some of the at least 75 wells; and d) a dispense map file based on the captured image that provides instructions to said liquid dispensing system to only dispense a reagent into wells containing only one cell, wherein the cell suspension is prepared from a tissue. - View Dependent Claims (25)
-
-
26. A system comprising:
-
a) a multi-well device comprising at least 75 wells; b) a cell suspension comprising cells present at a concentration such that, on average, one cell is present in a dispense volume; c) a liquid dispensing system that dispenses said dispense volume of said cell suspension into each of said at least 75 wells so that each of the at least 75 wells contains one cell, zero cells or more than one cell and at least some of the wells contain zero cells; d) an image capturing system configured to capture an image of at least some of the at least 75 wells; and e) a dispense map file based on the captured image that provides instructions to said liquid dispensing system to only dispense a reagent into wells containing only one cell, wherein the cells of the cell suspension are labeled with a first detectable label, a second detectable label, or both first and second detectable labels.
-
Specification