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Method for rapid accurate dispensing, visualization and analysis of single cells

  • US 10,641,772 B2
  • Filed: 02/20/2016
  • Issued: 05/05/2020
  • Est. Priority Date: 02/20/2015
  • Status: Active Grant
First Claim
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1. A method comprising:

  • a) dispensing with a liquid dispensing system a dispense volume of a cell suspension into each of at least 75 wells in a multi-well device so that each of the at least 75 wells contains one cell, zero cells or more than one cell and at least some of the wells contain zero cells, wherein said cell suspension comprises cells present at a concentration such that, on average, one cell is present in said dispense volume;

    b) capturing a digital image of at least some of the at least 75 wells using an image capturing system;

    c) generating a dispense map from the digital image and storing the dispense map to a computer memory, wherein the dispense map provides instructions to the liquid dispensing system to only dispense a reagent into wells containing only one cell; and

    d) dispensing, with the liquid dispensing system, the reagent into wells containing only one cell based on the generated dispense map stored in the computer memory.

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