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Methods to select for agents that stabilize protein complexes

  • US 10,641,779 B2
  • Filed: 07/17/2015
  • Issued: 05/05/2020
  • Est. Priority Date: 07/22/2014
  • Status: Active Grant
First Claim
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1. A method for selecting a conformation-selective binding agent of a transient protein complex comprising a conformational epitope, the method comprising the steps of:

  • a) displaying a collection of binding agents at the extracellular surfaces of a population of cells; and

    b) utilizing cell sorting to separate and select, from the population of cells, cells displaying binding agents thati. specifically bind to a conformational epitope of the transient protein complex and not to the individual members of the transient protein complex, and/orii. specifically bind to a conformational epitope of one of the individual members of the transient protein complex and not to a conformational epitope of the transient protein complex nor to the other individual member(s) of the transient protein complex, and/oriii. specifically bind to a conformational epitope of one of the individual members of the transient protein complex and to a conformational epitope of the transient protein complex and not to the other individual member(s) of the transient protein complex;

    wherein the transient protein complex comprises at least two interacting individual members; and

    wherein the individual members of the transient protein complex are distinguishably tagged.

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