Light sheet microscope with a phase-selective element for illumination
First Claim
1. A light sheet microscope, comprising:
- an illumination apparatus that generates coherent illumination light having several illumination wavelengths;
a beam-shaping module that generates a light sheet from the illumination light;
an illumination objective for illumination of a specimen with the light sheet;
a detection objective for imaging light emitted by the specimen onto a laminar detector, wherein an optical axis of the detection objective forms an angle different from 0° and
180°
with an optical axis of the illumination objective;
wherein the beam-shaping module comprises a phase-selective element arranged in or near an intermediate plane and having a plurality of selection areas that are separated from each other spatially;
wherein one selection area of the plurality of selection areas is assigned to one specific illumination wavelength of the several illumination wavelengths;
wherein a phase distribution predefined for the one specific illumination wavelength is impressed on the one selection area;
wherein the beam-shaping module comprises selecting means for sequentially or simultaneously selecting the selection areas in dependence on the respective illumination wavelength; and
wherein the selecting means is formed to direct light of the respective illumination wavelength onto an assigned selection area.
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Accused Products
Abstract
A light sheet microscope which includes an illumination apparatus generating coherent illumination light for several illumination wavelengths, a beam-shaping module generating a light sheet from illumination light, an illumination objective illuminating a specimen with the light sheet and a detection objective for imaging light which is emitted by the specimen onto a laminar detector, wherein the optical axes of the detection objective and of the illumination objective are not parallel to each other. In such a light sheet microscope, the beam-shaping module includes a phase-selective element with several selection areas separated from each other spatially, wherein in each case one selection area is assigned to one specific illumination wavelength, and wherein a phase distribution predefined for the respective illumination wavelength is impressed on each selection area. The beam-shaping module further includes means for the sequential or simultaneous selection of the selection areas in dependence on the respective illumination wavelength.
9 Citations
25 Claims
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1. A light sheet microscope, comprising:
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an illumination apparatus that generates coherent illumination light having several illumination wavelengths; a beam-shaping module that generates a light sheet from the illumination light; an illumination objective for illumination of a specimen with the light sheet; a detection objective for imaging light emitted by the specimen onto a laminar detector, wherein an optical axis of the detection objective forms an angle different from 0° and
180°
with an optical axis of the illumination objective;wherein the beam-shaping module comprises a phase-selective element arranged in or near an intermediate plane and having a plurality of selection areas that are separated from each other spatially; wherein one selection area of the plurality of selection areas is assigned to one specific illumination wavelength of the several illumination wavelengths; wherein a phase distribution predefined for the one specific illumination wavelength is impressed on the one selection area; wherein the beam-shaping module comprises selecting means for sequentially or simultaneously selecting the selection areas in dependence on the respective illumination wavelength; and wherein the selecting means is formed to direct light of the respective illumination wavelength onto an assigned selection area. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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16. A method for examining a specimen by light sheet microscopy, comprising:
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selecting several illumination wavelengths for composing illumination light for the specimen; impressing a predefined phase distribution on a phase-selective element for each of the illumination wavelengths, wherein each of the predefined phase distributions is impressed on the phase-selective element in its own selection area, which is assigned to the respective illumination wavelength and does not overlap with the other selection areas; illuminating the phase-selective element with illumination light in or near to an intermediate image plane in an illumination beam path, wherein only light of the respective illumination wavelength is directed onto each selection area and structured by the phase-selective element before the light of the different illumination wavelengths is combined to form a common beam, shaping, in a focal plane of a downstream illumination objective, a structured light sheet of the respective illumination wavelength with a light sheet plane which is perpendicular to a focal plane of the illumination objective, and illuminating the specimen with the structured light sheets of the respective illumination wavelengths in the light sheet plane, and detecting light emitted by the specimen in a detection direction which forms an angle different from zero with the light sheet plane. - View Dependent Claims (17, 18, 19, 20, 21, 22, 23, 24, 25)
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Specification