Imaging device for microscope

• US 10,642,018 B2
• Filed: 09/06/2016
• Issued: 05/05/2020
• Est. Priority Date: 03/06/2014
• Status: Active Grant

• Current Assignee: ACQUIFER Imaging GmbH (Bruker Corporation), Karlsruher Institut Fur Technologie
• Original Assignee: Karlsruher Institut Fur Technologie
• Inventors: Hufnagel, Lars, Krzic, Uros, Liebel, Urban
• Primary Examiner(s): Rakowski, Cara E
• Application Number:
  US15/256,981
• Publication Number:
  US 20160377852A1
• Time in Patent Office: 1,337 Days
• CPC Class Codes: G02B 21/06 Means for illuminating specimens
• CPC Class Codes: G02B 21/16 adapted for ultraviolet illumination ; Fluorescence microscopes G02B21/0076 takes precedence
• CPC Class Codes: G02B 21/26 Stages; Adjusting means therefor
• CPC Class Codes: G02B 21/28 with cooling device
• CPC Class Codes: G02B 21/362 Mechanical details, e.g. mountings for the camera or image sensor, housings G02B21/364 takes precedence

Abstract

An imaging device for a microscope (20) is disclosed that comprises a plurality of individual light sources (40) and a detector (15). The plurality of the individual light sources (40) is mounted on a translation stage (110). The imaging device further includes moveable optics arranged to direct a light path (45) onto a stationary sample (60) and collect reflected or fluoresced radiation from the sample (60).

Claims

1. A microscope comprising:

a support member;

a first stage movably mounted to said support member, said first stage being movable relative to said support member in a first direction;

a second stage movably mounted to said first stage, said second stage being movable relative to said first stage in a second direction different than said first direction;

an objective lens mounted to said second stage;

an illumination device movably mounted to said second stage, said illumination device being movable on said second stage relative to said objective lens in a third direction, said illumination device comprising;

a plurality of individual light sources for illuminating at least one stationary sample through said objective lens, wherein different ones of said plurality of individual light sources can be aligned with said objective lens by moving said illumination device relative to said objective lens in said third direction, wherein said third direction is the same as one of said first and second directions; and

a detector for detecting radiation from an illuminated one of the at least one stationary sample;

wherein the illumination device, the objective lens, and the detector are movable in a plane formed by said first direction and said second direction.

2. The microscope of claim 1, further comprising a Peltier device, arranged between at least one of the plurality of individual light sources and a heat sink, on which the plurality of individual light sources is mounted.

3. The microscope of claim 1, further comprising a band pass filter arranged in the light path between the at least one of the plurality of individual light sources and the stationary sample.

4. The microscope of claim 3, further comprising a diffuser being arranged in the light path between the band pass filter and the stationary sample.

5. The microscope of claim 1, wherein the individual light sources are light emitting diodes.

6. The microscope of claim 1, further comprising a plurality of translation rails cooperating with at least one motor to move the illumination device, the objective lens, and the detector with respect to the stationary sample.

7. The microscope of claim 1, wherein the second stage is movably mounted on the first stage in the first direction, the first stage being movably mounted on the support member in the second direction, and

the illumination device is movable with respect to the second stage in the second direction.

8. The microscope according to claim 1, wherein said illumination device further comprises:

a spectral filter associated with each of said individual light sources, each said spectral filter being different.