Raman cluster tagged molecules for biological imaging
First Claim
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1. A nucleoside polyphosphate analog having the structure:
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Abstract
This invention provides nucleoside polyphosphate analogs each of which comprises a tag comprising a plurality of Raman-scattering moieties; compounds comprising said nucleoside polyphosphate analogs; and methods for determining the sequence of a single-stranded DNA or RNA using said nucleoside polyphosphate analogs. This invention also provides methods for detecting the interaction of a plurality of predetermined compounds, at least one of which having attached thereto a tag comprising a plurality of Raman-scattering moieties.
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20 Claims
- 1. A nucleoside polyphosphate analog having the structure:
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11. A composition comprising four deoxyribonucleoside polyphosphate (dNPP) analogs, each dNPP analog having the structure:
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12. A composition comprising four ribonucleoside polyphosphate (rNPP) analogs, each rNPP analog having the structure:
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13. A method for determining the sequence of a single-stranded DNA comprising:
(a) contacting the single-stranded DNA having a primer hybridized to a portion thereof with a DNA polymerase and four deoxyribonucleoside polyphosphate (dNPP) analogs under conditions permitting the DNA polymerase to catalyze incorporation onto the primer of a dNPP analog complementary to a nucleotide residue of the single-stranded DNA which is immediately 5′
to a nucleotide residue of the single-stranded DNA hybridized to the 3′
terminal nucleotide residue of the primer, so as to form a DNA extension product, wherein each dNPP analog has the structure;- View Dependent Claims (15, 16, 17, 18, 19, 20)
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14. A method for determining the sequence of a single-stranded RNA comprising:
(a) contacting the single-stranded RNA having a primer hybridized to a portion thereof with an RNA polymerase and four ribonucleoside polyphosphate (rNPP) analogs under conditions permitting the RNA polymerase to catalyze incorporation onto the primer of an rNPP analog complementary to a nucleotide residue of the single-stranded RNA which is immediately 5′
to a nucleotide residue of the single-stranded RNA hybridized to the 3′
terminal nucleotide residue of the primer, so as to form an RNA extension product, wherein each rNPP analog has the structure;
Specification