Method for isolating cross-reactive aptamer and use thereof
First Claim
1. An electrochemical aptamer-based (E-AB) sensor comprising a structure-switching cross-reactive aptamer and an electrode, the structure-switching cross-reactive aptamer being conjugated to the surface of the electrode, the structure-switching cross-reactive aptamer comprising SEQ ID No:
- 19.
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Abstract
The subject invention provides a novel SELEX strategy for isolating cross-reactive aptamers that recognize a core structure of a small-molecule family and bind to each structurally-similar molecule in said family. The subject invention also provides methods, assays, and products for detecting small-molecule targets of the family in a sample in both clinical and field settings. Such method is based on an aptamer sensor that reports the presence of small-molecule targets via a sensitive colorimetric signal for naked-eye detection. The subject invention further provides exonuclease-based methods for generating structure-switching aptamers from fully folded aptamers and developing electrochemical aptamer-based (E-AB) sensors for rapid and sensitive detection of synthetic cathinones.
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Citations
17 Claims
- 1. An electrochemical aptamer-based (E-AB) sensor comprising a structure-switching cross-reactive aptamer and an electrode, the structure-switching cross-reactive aptamer being conjugated to the surface of the electrode, the structure-switching cross-reactive aptamer comprising SEQ ID No:
Specification