Rare event detection using mass tags
First Claim
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1. A method for detecting a target analyte in a sample, the method comprising:
- conducting an assay that specifically associates a mass tag with a target analyte in a sample;
depolymerizing the mass tag to generate a plurality of molecules that are dissociated from the target analyte;
capturing the plurality of molecules via an affinity agent on a spray probe, thereby pre-concentrating the plurality of molecules;
generating, via the spray probe, a plurality of ions from the plurality of molecules; and
analyzing the plurality of ions, thereby detecting the target analyte from the sample.
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Abstract
The invention generally relates to methods for rare event detection using mass tags. In certain embodiments, the invention provides methods for detecting a target analyte in a sample that involve conducting an assay that specifically associates a mass tag with a target analyte in a sample, generating ions of the mass tag, and analyzing the generated ions of the mass tag, thereby detecting the target analyte from the sample.
13 Citations
20 Claims
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1. A method for detecting a target analyte in a sample, the method comprising:
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conducting an assay that specifically associates a mass tag with a target analyte in a sample; depolymerizing the mass tag to generate a plurality of molecules that are dissociated from the target analyte; capturing the plurality of molecules via an affinity agent on a spray probe, thereby pre-concentrating the plurality of molecules; generating, via the spray probe, a plurality of ions from the plurality of molecules; and analyzing the plurality of ions, thereby detecting the target analyte from the sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A method for detecting a target analyte in a sample, the method comprising:
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applying a voltage to a spray probe to generate a plurality of ions from a plurality of molecules, the plurality of molecules being generated from depolymerization of a mass tag that is specifically associated with a target analyte from a sample wherein the plurality of molecules are dissociated from the target analyte, and are captured via an affinity agent on the spray probe, thereby pre-concentrating the plurality of molecules; and analyzing the plurality of ions from the plurality of molecules, thereby detecting the target analyte from the sample. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18, 19, 20)
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Specification