Spatially encoded biological assays
First Claim
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1. A method of associating presence or abundance of a target biological molecule with a location of a tissue sample, comprising:
- delivering a plurality of probes to a tissue sample affixed to a support, wherein a probe of the plurality of probes comprises a protein that specifically binds a target biological molecule of the tissue sample, wherein the protein is conjugated to an oligonucleotide having a sequence, andseparating the probe specifically bound to the target biological molecule at the location of the tissue sample from the plurality of probes not specifically bound to the target biological molecule at the location of the tissue sample; and
sequencing all or a portion of the sequence of the oligonucleotide or a complement thereof, from the specifically bound probe, and using the determined sequence to associate presence or abundance of the target biological molecule with the location of the tissue sample.
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Abstract
The present invention provides assays and assay systems for use in spatially encoded biological assays. The invention provides an assay system comprising an assay capable of high levels of multiplexing where reagents are provided to a biological sample in defined spatial patterns; instrumentation capable of controlled delivery of reagents according to the spatial patterns; and a decoding scheme providing a readout that is digital in nature.
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Citations
30 Claims
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1. A method of associating presence or abundance of a target biological molecule with a location of a tissue sample, comprising:
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delivering a plurality of probes to a tissue sample affixed to a support, wherein a probe of the plurality of probes comprises a protein that specifically binds a target biological molecule of the tissue sample, wherein the protein is conjugated to an oligonucleotide having a sequence, and separating the probe specifically bound to the target biological molecule at the location of the tissue sample from the plurality of probes not specifically bound to the target biological molecule at the location of the tissue sample; and sequencing all or a portion of the sequence of the oligonucleotide or a complement thereof, from the specifically bound probe, and using the determined sequence to associate presence or abundance of the target biological molecule with the location of the tissue sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30)
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Specification