Molecular markers associated with soybean tolerance to low iron growth conditions

• US 10,662,486 B2
• Filed: 07/16/2018
• Issued: 05/26/2020
• Est. Priority Date: 06/10/2013
• Status: Active Grant

• Current Assignee: Monsanto Technology LLC (Bayer AG)
• Original Assignee: Monsanto Technology LLC (Bayer AG)
• Inventors: Jia, Hongwu, La Vallee, Bradley, Lussenden, Roger L., Yates, Jennifer L., Ye, Xianghai
• Primary Examiner(s): Stankovic, Bratislav
• Application Number:
  US16/036,361
• Publication Number:
  US 20180312932A1
• Time in Patent Office: 680 Days
• Field of Search: None
• CPC Class Codes: A01H 1/045 using molecular markers
• CPC Class Codes: C12Q 1/6895 for plants, fungi or algae
• CPC Class Codes: C12Q 2600/13 Plant traits
• CPC Class Codes: Y10T 436/143333 Saccharide [e.g., DNA, etc.]

Abstract

The present invention provides methods and compositions for identifying soybean plants that are tolerant or have improved tolerance, or those that are susceptible to, iron deficient growth conditions. The methods use molecular markers to identify, select, and/or introgress genetic loci modulating phenotypic expression of an iron deficiency tolerance trait in soybean plant breeding. Methods are provided for screening germplasm entries for the performance and expression of this trait.

Claims

1. A method of creating a population of soybean plants with a low iron growth condition tolerant phenotype, comprising:

a. genotyping a first population of soybean plants or seeds;

b. detecting in said first population of soybean plants or seeds at least one polymorphic nucleic acid marker linked by less than or equal to 10 cM to an allele of a sequence selected from the group consisting of SEQ ID NOs;

71 to 82, wherein said allele is associated with a low iron growth condition tolerant phenotype;

c. selecting based upon said genotyping a soybean plant or seed containing said allele;

d. crossing or selfing said selected soybean plant or a plant produced from said selected seed; and

e. producing from said crossing or selfing a population comprising at least one progeny soybean plant comprising said allele and a low iron growth condition tolerant phenotype;

wherein said allele of SEQ ID NO;

71 comprises an adenosine at position 650,

wherein said allele of SEQ ID NO;

72 comprises an adenosine at position 201,

wherein said allele of SEQ ID NO;

73 comprises an tyrosine at position 201,

wherein said allele of SEQ ID NO;

74 comprises an guanine at position 201,

wherein said allele of SEQ ID NO;

75 comprises an guanine at position 201,

wherein said allele of SEQ ID NO;

76 comprises an guanine at position 201,

wherein said allele of SEQ ID NO;

77 comprises an tyrosine at position 201,

wherein said allele of SEQ ID NO;

78 comprises an guanine at position 201,

wherein said allele of SEQ ID NO;

79 comprises an tyrosine at position 201,

wherein said allele of SEQ ID NO;

80 comprises an tyrosine at position 201,

wherein said allele of SEQ ID NO;

81 comprises an guanine at position 201, and

wherein said allele of SEQ ID NO;

82 comprises an tyrosine at position 201.

2. The method of claim 1, wherein said polymorphic nucleic acid marker is genetically linked by less than or equal to 5 cM to said allele of a sequence selected from the group consisting of SEQ ID NOs:

71 to 82.

3. The method of claim 1, wherein said polymorphic nucleic acid marker is genetically linked by less than or equal to 10 cM to said allele of a sequence selected from the group consisting of SEQ ID NOs:

71 to 79.

4. The method of claim 1, wherein said polymorphic nucleic acid marker is flanked by SEQ ID NO:

71 and SEQ ID NO;

82.

5. The method of claim 1, wherein said polymorphic nucleic acid marker is genetically linked by less than or equal to 5 cM to said allele of a sequence selected from the group consisting of SEQ ID NOs:

71 to 79.

6. The method of claim 1, wherein said polymorphic nucleic acid marker is from a chromosome region flanked by any two sequences selected from the group consisting of SEQ ID NOs:

71 to 82.

7. The method of claim 1, wherein said marker locus is of a marker type selected from the group consisting of Restriction Fragment Length Polymorphisms (RFLP), Amplified Fragment Length Polymorphisms (AFLP), Simple Sequence Repeats (SSR), Single Nucleotide Polymorphisms (SNP), Insertion/Deletion Polymorphisms (Indels), Variable Number Tandem Repeats (VNTR), and Random Amplified Polymorphic DNA (RAPD).

8. The method of claim 1, wherein said detecting an allele comprises a method selected form the group consisting of a PCR-based detection method, a microarray method, a mass spectrometry-based method, and a nucleic acid sequencing method.

9. The method of claim 1, wherein said detecting an allele comprises a single base extension (SBE) assay.

10. The method of claim 1, wherein said molecular marker is within 1 cM from said allele of a sequence selected from the group consisting of SEQ ID NOs:

71 to 82.

11. The method of claim 1, wherein said allele comprises SEQ ID NO:

78.

12. The method of claim 1, wherein said allele comprises SEQ ID NO:

79.

13. The method of claim 1, wherein said polymorphic nucleic acid marker is within 10 cM to said allele of SEQ ID NO:

78.

14. The method of claim 1, wherein said polymorphic nucleic acid marker is within 10 cM to said allele of SEQ ID NO:

79.

15. The method of claim 1, wherein said allele comprises SEQ ID NO:

77.

16. The method of claim 1, wherein said polymorphic nucleic acid marker is within 10 cM to said allele of SEQ ID NO:

77.

17. The method of claim 1, wherein said molecular marker is within 1 cM from said allele of a sequence selected from the group consisting of SEQ ID NOs:

71 to 79.

18. The method of claim 1, wherein said at least one polymorphic nucleic acid marker comprises a sequence selected from group consisting of SEQ ID NOs:

71 to 82.

19. The method of claim 1, wherein said first population of soybean plants or seeds comprises at least one soybean plant or a plant grown from said seeds comprising a low iron growth condition tolerant phenotype.