Methods of manufacturing injectable microgel scaffolds
First Claim
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1. A method comprising:
- a) providing a membrane filtration system comprising a solid support with pores, each pore having a diameter of at most 9 micrometers (μ
m);
b) transferring dispersed microgel particles from a first continuous phase to a final continuous phase by controlled addition of an intermediate continuous phase to the first continuous phase, wherein the final continuous phase is immiscible with the first continuous phase;
c) maintaining a single miscible continuous phase containing the dispersed microgel particles;
d) applying the single miscible continuous phase containing the dispersed microgel particles to a membrane of the membrane filtration system; and
e) removing an impurity from the dispersed microgel particles or the single miscible continuous phase using size exclusion filtration by the membrane filtration system, thereby producing purified microgel particles.
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Abstract
Disclosed herein are methods of manufacturing injectable microgel scaffolds, including methods of producing, purifying and concentrating microgel particles therein. The microgel scaffolds of the present disclosure are useful for a wide range of applications, such as stabilizing an implanted medical device in an implant site in a subject. The microgel scaffolds are fluidic during application and annealed or crosslinked after application to the implant site in the subject. The microgel scaffolds may contain various therapeutic agents, including antibiotics and analgesics, throughout the gel.
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Citations
20 Claims
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1. A method comprising:
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a) providing a membrane filtration system comprising a solid support with pores, each pore having a diameter of at most 9 micrometers (μ
m);b) transferring dispersed microgel particles from a first continuous phase to a final continuous phase by controlled addition of an intermediate continuous phase to the first continuous phase, wherein the final continuous phase is immiscible with the first continuous phase; c) maintaining a single miscible continuous phase containing the dispersed microgel particles; d) applying the single miscible continuous phase containing the dispersed microgel particles to a membrane of the membrane filtration system; and e) removing an impurity from the dispersed microgel particles or the single miscible continuous phase using size exclusion filtration by the membrane filtration system, thereby producing purified microgel particles. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 20)
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- 9. The method of 1, wherein the purified microgel particles comprise a backbone polymer and annealing components.
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