Methods and devices for de novo oligonucleic acid assembly
First Claim
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1. A method for preparing a surface for polynucleotide synthesis, the method comprising:
- depositing a first population of a first plurality of molecules on a surface of a solid support at a first region, wherein the first region comprises a plurality of loci, and wherein the first plurality of molecules comprises a plurality of non-coupling molecules that bind to the surface and lack a reactive group capable of binding to a nucleoside; and
depositing a mixture on the solid support at the first region, wherein the mixture comprises a second population of the first plurality of molecules and a second plurality of molecules, and wherein the second plurality of molecules comprises a plurality of coupling molecules that bind to the solid support and comprise the reactive group that is capable of binding to the nucleoside, and wherein the first plurality of molecules and the second plurality of molecules are present in the mixture in a molar ratio of 10;
1 to 2500;
1.
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Abstract
Methods and devices are provided herein for surfaces for de novo nucleic acid synthesis which provide for low error rates. In addition, methods and devices are provided herein for increased nucleic acid mass yield resulting from de novo nucleic acid synthesis.
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Citations
20 Claims
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1. A method for preparing a surface for polynucleotide synthesis, the method comprising:
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depositing a first population of a first plurality of molecules on a surface of a solid support at a first region, wherein the first region comprises a plurality of loci, and wherein the first plurality of molecules comprises a plurality of non-coupling molecules that bind to the surface and lack a reactive group capable of binding to a nucleoside; and depositing a mixture on the solid support at the first region, wherein the mixture comprises a second population of the first plurality of molecules and a second plurality of molecules, and wherein the second plurality of molecules comprises a plurality of coupling molecules that bind to the solid support and comprise the reactive group that is capable of binding to the nucleoside, and wherein the first plurality of molecules and the second plurality of molecules are present in the mixture in a molar ratio of 10;
1 to 2500;
1. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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Specification