Antibodies comprising site-specific non-natural amino acid residues, methods of their preparation and methods of their use
First Claim
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1. An isolated antibody of the IgG class comprising a heavy chain and a light chain, wherein:
- the heavy chain comprises at least one non-natural amino acid residue at a specific site selected from the group consisting of heavy chain residue 52 according to the Kabat numbering scheme and heavy chain residues 119, 222, and 241 according to the EU index of Kabat;
orthe light chain comprises a non-natural amino acid residue at the light chain residue 152;
or a combination thereof;
or an aglycosylated variant thereof,wherein each non-natural amino acid residue is independently selected from the group consisting of ortho-substituted tyrosine, meta substituted tyrosine, para-substituted phenylalanine, ortho-substituted phenylalanine, and meta-substituted phenylalanine.
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Abstract
Provided herein are antibodies comprising non-natural amino acid residues at site-specific positions, compositions comprising the antibodies, methods of their production and methods of their use. The antibodies are useful for methods of treatment and prevention, methods of detection and methods of diagnosis.
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16 Claims
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1. An isolated antibody of the IgG class comprising a heavy chain and a light chain, wherein:
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the heavy chain comprises at least one non-natural amino acid residue at a specific site selected from the group consisting of heavy chain residue 52 according to the Kabat numbering scheme and heavy chain residues 119, 222, and 241 according to the EU index of Kabat;
orthe light chain comprises a non-natural amino acid residue at the light chain residue 152;
or a combination thereof;or an aglycosylated variant thereof, wherein each non-natural amino acid residue is independently selected from the group consisting of ortho-substituted tyrosine, meta substituted tyrosine, para-substituted phenylalanine, ortho-substituted phenylalanine, and meta-substituted phenylalanine. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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Specification