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Massive parallel method for decoding DNA and RNA

  • US 10,669,577 B2
  • Filed: 03/27/2015
  • Issued: 06/02/2020
  • Est. Priority Date: 10/06/2000
  • Status: Expired due to Term
First Claim
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1. A method for simultaneously sequencing different deoxyribonucleic acid templates immobilized on a solid surface, which comprises (1) simultaneously applying to said immobilized different deoxyribonucleic acid templates a sequencing by synthesis method comprising multiple cycles, and (2) detecting the identity of the nucleotide analogue incorporated onto each growing DNA strand hybridized to each of said different deoxyribonucleic acid templates, each cycle comprises the following steps:

  • (a) providing to the different deoxyribonucleic acid templates at least one nucleotide analogue having the structure;

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