Massive parallel method for decoding DNA and RNA
First Claim
1. A method for simultaneously sequencing different deoxyribonucleic acid templates immobilized on a solid surface, which comprises (1) simultaneously applying to said immobilized different deoxyribonucleic acid templates a sequencing by synthesis method comprising multiple cycles, and (2) detecting the identity of the nucleotide analogue incorporated onto each growing DNA strand hybridized to each of said different deoxyribonucleic acid templates, each cycle comprises the following steps:
- (a) providing to the different deoxyribonucleic acid templates at least one nucleotide analogue having the structure;
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Abstract
This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.
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13 Claims
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1. A method for simultaneously sequencing different deoxyribonucleic acid templates immobilized on a solid surface, which comprises (1) simultaneously applying to said immobilized different deoxyribonucleic acid templates a sequencing by synthesis method comprising multiple cycles, and (2) detecting the identity of the nucleotide analogue incorporated onto each growing DNA strand hybridized to each of said different deoxyribonucleic acid templates, each cycle comprises the following steps:
(a) providing to the different deoxyribonucleic acid templates at least one nucleotide analogue having the structure; - View Dependent Claims (2, 3, 4, 5, 6, 7, 12)
- 8. A method for sequencing a nucleic acid which comprises detecting the identity of a nucleotide analogue incorporated onto the end of a growing DNA strand in a polymerase reaction, wherein the nucleotide analogue is any of the following:
Specification