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DNA sequencing system with stacked BSI global shutter image sensor

  • US 10,670,526 B2
  • Filed: 03/05/2018
  • Issued: 06/02/2020
  • Est. Priority Date: 03/05/2018
  • Status: Active Grant
First Claim
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1. An apparatus for identifying the presence of fluorophore tagged nucleotide bases in a sample, the apparatus comprising:

  • a laser illumination source configured to emit pulses of radiation;

    an optically transparent sample holding chamber adapted to receive the sample, the sample holding chamber having a transparent top side to receive the pulses of radiation from the laser illumination source into the sample holding chamber, in order to identify the presence of the fluorophore tagged nucleotide bases by imaging the fluorescent emissions which may persist after the laser illumination ceases;

    an image sensor chip including an array of image sensing pixels with its image sensing surface stacked immediately adjacent and under the sample holding chamber and configured with in-pixel global shutter for accumulating and transferring an image signal in response to persistent fluorescent emission light incident upon the image sensor;

    a circuit chip with circuit blocks disposed within a second substrate stacked immediately adjacent and upon the other surface of the image sensor chip for coupling out the image signal through the second substrate to information processing and analysis component circuit blocks;

    wherein the image sensor global shutter pixels are open to accumulate persistent fluorescent emission and transfer out a related image signal when no laser radiation is directed into the sample holding chamber; and

    wherein the image sensor global shutter pixels are closed or in reset mode when the laser radiation pulse is directed into the sample holding chamber.

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