Method and system for protecting honey bees from fipronil pesticides

• US 10,675,347 B2
• Filed: 01/14/2019
• Issued: 06/09/2020
• Est. Priority Date: 11/30/2015
• Status: Active Grant

• Current Assignee: Seed Health, Inc.
• Original Assignee: Joseph E. Kovarik
• Inventors: Kovarik, Joseph E.
• Primary Examiner(s): Ogunbiyi, Oluwatosin A
• Application Number:
  US16/246,652
• Publication Number:
  US 20190142934A1
• Time in Patent Office: 512 Days
• Field of Search: None
• CPC Class Codes: A61K 2039/505 comprising antibodies
• CPC Class Codes: A61K 2039/541 Mucosal route
• CPC Class Codes: A61K 2039/544 to the airways intranasal A61K2039/543
• CPC Class Codes: A61K 2039/55 characterised by the host/recipient, e.g. newborn with maternal antibodies
• CPC Class Codes: A61K 2039/55583 Polysaccharides
• CPC Class Codes: A61K 2039/577 tolerising response
• CPC Class Codes: A61K 2039/58 raising an immune response against a target which is not the antigen used for immunisation
• CPC Class Codes: A61K 2039/70 Multivalent vaccine
• CPC Class Codes: A61K 35/74 Bacteria therapeutic use of a bacterial protein A61K38/00
• CPC Class Codes: A61K 35/741 Probiotics probiotic yeast, e.g. saccharomyces A61K36/06
• CPC Class Codes: A61K 35/742 Spore-forming bacteria, e.g. Bacillus coagulans, Bacillus subtilis, clostridium or Lactobacillus sporogenes
• CPC Class Codes: A61K 35/745 Bifidobacteria
• CPC Class Codes: A61K 35/747 Lactobacilli, e.g. L. acidophilus or L. brevis
• CPC Class Codes: A61K 39/0008 Antigens related to auto-immune diseases; Preparations to induce self-tolerance
• CPC Class Codes: A61K 39/35 Allergens
• CPC Class Codes: A61K 39/39 characterised by the immunostimulating additives, e.g. chemical adjuvants
• CPC Class Codes: A61K 39/395 Antibodies agglutinins A61K38/36 ; as drug carriers A61K47/50; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
• CPC Class Codes: A61K 45/06 Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
• CPC Class Codes: A61K 9/0034 Urogenital system, e.g. vagina, uterus, cervix, penis, scrotum, urethra, bladder; Personal lubricants
• CPC Class Codes: A61K 9/0043 Nose
• CPC Class Codes: A61K 9/007 : Pulmonary tract; Aromatherapy
• CPC Class Codes: C07K 16/00 : Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies antibodies with enzymatic activity, e.g. abzymes C12N9/0002

Abstract

A method and system for the treatment of honey bees (Apis mellifera), bats, and butterflies protects them from various life threatening conditions, including Colony Collapse Disorder, white nose syndrome, etc. and in particular, provides honey bees, bats and butterflies with the ability to assimilate and degrade pesticides such as neonicotinoids and fipronil.

Claims

1. A method for providing a honey bee with the ability to assimilate fipronil pesticides, comprising, inoculating a honey bee with a culture of fipronil degrading bacteria, wherein the fipronil degrading bacteria comprises at least one of, Paracoccus denitrificans, and Stenotrophomonas acidaminiphila.

2. The method as set forth in claim 1, wherein the culture comprises a fipronil degrading bacteria comprising Paracoccus denitrificans.

3. The method as set forth in claim 1, wherein the culture comprises a fipronil degrading bacteria that comprises Stenotrophomonas acidaminiphila.

4. The method as set forth in claim 1, wherein the culture comprises a fipronil degrading bacteria that comprises both of Paracoccus denitrificans and Stenotrophomonas acidaminiphila.

5. A method for providing a honey bee with the ability to assimilate fipronil pesticides, comprising, inoculating a honey bee with a culture of pesticide degrading bacteria, wherein the pesticide comprises fipronil, and said culture comprises Paracoccus denitrificans.

6. The method as set forth in claim 5, wherein the culture further comprises one of Stenotrophomonas acidaminiphila, and Bacillus thuringiensis.

7. The method as set forth in claim 5, wherein the culture further comprises Stenotrophomonas acidaminiphila.

8. A method for providing a honey bee with the ability to assimilate fipronil pesticides, comprising, inoculating the gut of a honey bee with microbes possessing the ability to degrade fipronil pesticides, said microbes comprising Paracoccus denitrificans and Bacillus thuringiensis.

9. The method as set forth in claim 8, wherein said microbes further comprises Stenotrophomonas acidaminiphila and Bacillus thuringiensis.