Compositions and methods for epigenome editing
First Claim
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1. A fusion protein comprising two heterologous polypeptide domains, wherein the first polypeptide domain comprises a Clustered Regularly Interspaced Short Palindromic Repeats associated (Cas) protein, wherein the second polypeptide domain comprises a p300 histone acetyltransferase effector domain, wherein the fusion protein activates transcription of a target gene by activating distal regulatory elements, and wherein the fusion protein comprises the polypeptide sequence of SEQ ID NO:
- 140, SEQ ID NO;
141, or SEQ ID NO;
149.
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Abstract
Disclosed herein are CRISPR/Cas9-based gene activation systems that include a fusion protein of a Cas9 protein and a protein having histone acetyltransferase activity, and methods of using said systems.
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4 Claims
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1. A fusion protein comprising two heterologous polypeptide domains, wherein the first polypeptide domain comprises a Clustered Regularly Interspaced Short Palindromic Repeats associated (Cas) protein, wherein the second polypeptide domain comprises a p300 histone acetyltransferase effector domain, wherein the fusion protein activates transcription of a target gene by activating distal regulatory elements, and wherein the fusion protein comprises the polypeptide sequence of SEQ ID NO:
- 140, SEQ ID NO;
141, or SEQ ID NO;
149. - View Dependent Claims (2)
- 140, SEQ ID NO;
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3. A fusion protein comprising two heterologous polypeptide domains,
wherein the first polypeptide domain comprises a Clustered Regularly Interspaced Short Palindromic Repeats associated (Cas) protein, wherein the second polypeptide domain comprises a p300 histone acetyltransferase effector domain, wherein the fusion protein activates transcription of a target gene by activating distal regulatory elements, and wherein the first polypeptide domain comprises the polypeptide sequence of SEQ ID NO: - 10 and the second polypeptide domain comprises the polypeptide sequence of SEQ ID NO;
3, or the first polypeptide domain comprises the polypeptide sequence of SEQ ID NO;
1 and the second polypeptide domain comprises the polypeptide sequence of SEQ ID NO;
2. - View Dependent Claims (4)
- 10 and the second polypeptide domain comprises the polypeptide sequence of SEQ ID NO;
Specification