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Models for analyzing data from sequencing-by-synthesis operations

  • US 10,679,724 B2
  • Filed: 04/27/2017
  • Issued: 06/09/2020
  • Est. Priority Date: 05/11/2012
  • Status: Active Grant
First Claim
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1. A method of modeling a background signal when sequencing a polynucleotide strand using sequencing-by-synthesis, comprising:

  • flowing a series of nucleotide flows onto a reactor array having multiple reaction confinement regions, one or more copies of the polynucleotide strand being located in a loaded reaction confinement region of the reactor array, the loaded reaction confinement region being located in a vicinity of one or more neighboring reaction confinement regions;

    receiving output signals from the reactor array; and

    estimating a background signal for the loaded reaction confinement region using the received output signals and a background model representing at least an exchange of ions between the one or more neighboring reaction confinement regions and a headspace adjacent to the loaded reaction confinement region and the one or more neighboring reaction confinement regions, wherein the background model is derived using a first characteristic equation, wherein the first characteristic equation comprises a term related to a difference between a proton concentration in each of the neighboring reaction confinement regions and a proton concentration in the headspace.

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