Structuring fats and methods of producing structuring fats
First Claim
1. A microbial oil comprising triglycerides, wherein the microbial oil has an altered fatty acid profile relative to a microbial oil produced by a non-recombinant oleaginous microbe, the microbial oil comprising at least 45% of triglycerides being a combination of palmitic-oleic-palmitic triglyceride, stearic-oleic-stearic triglyceride, and palmitic-oleic-stearic triglyceride, and wherein the oleaginous microbe comprises an exogenous gene encoding ketoacyl-ACP synthase (KAS).
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Abstract
Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desaturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsaturated-saturated type.
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10 Claims
- 1. A microbial oil comprising triglycerides, wherein the microbial oil has an altered fatty acid profile relative to a microbial oil produced by a non-recombinant oleaginous microbe, the microbial oil comprising at least 45% of triglycerides being a combination of palmitic-oleic-palmitic triglyceride, stearic-oleic-stearic triglyceride, and palmitic-oleic-stearic triglyceride, and wherein the oleaginous microbe comprises an exogenous gene encoding ketoacyl-ACP synthase (KAS).
Specification