Methods and compositions for preparing nucleic acid libraries
First Claim
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1. A method for preparing a nucleic acid library from a low quality nucleic acid sample comprising:
- (a) providing a plurality of nucleic acids obtained from the low quality nucleic acid sample; and
(b) selectively shearing the plurality of nucleic acids comprising;
(i) separating the plurality of nucleic acids into a first fraction of nucleic acids having an average length longer than 550 base pairs, and a second fraction of nucleic acids having an average length shorter than 550 base pairs,(ii) shearing the nucleic acids of the first fraction, and(iii) combining the first fraction and the second fraction, thereby preparing the nucleic acid library.
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Abstract
Embodiments provided herein relate to methods and compositions for preparing nucleic acid libraries. Some embodiments include preparing libraries from nucleic acids obtained from degraded samples, such as ancient samples and fixed samples.
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20 Claims
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1. A method for preparing a nucleic acid library from a low quality nucleic acid sample comprising:
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(a) providing a plurality of nucleic acids obtained from the low quality nucleic acid sample; and (b) selectively shearing the plurality of nucleic acids comprising; (i) separating the plurality of nucleic acids into a first fraction of nucleic acids having an average length longer than 550 base pairs, and a second fraction of nucleic acids having an average length shorter than 550 base pairs, (ii) shearing the nucleic acids of the first fraction, and (iii) combining the first fraction and the second fraction, thereby preparing the nucleic acid library. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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16. A method for preparing a nucleic acid library from a low quality nucleic acid sample comprising:
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(a) providing a plurality of nucleic acids obtained from the low quality nucleic acid sample; (b) selectively shearing the plurality of nucleic acids comprising; (i) separating the plurality of nucleic acids into a first fraction of nucleic acids having an average length longer than 550 base pairs, and a second fraction of nucleic acids having an average length shorter than 550 base pairs, (ii) shearing the nucleic acids of the first fraction, and (iii) combining the first fraction and the second fraction; and (c) repairing the nucleic acids of the combined fractions, thereby preparing the nucleic acid library. - View Dependent Claims (17, 18, 19, 20)
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Specification