Capture reactions
First Claim
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1. A method of improving performance of molecular inversion probe capture reactions, the method comprising:
- obtaining a nucleic acid;
fragmenting the nucleic acid into a plurality of nucleic acid fragments to make a target available for capture;
introducing a plurality of molecular inversion probes (MIPs) to the plurality of nucleic acid fragments, wherein each of the plurality of MIPs hybridize to the target and captures a different one of a plurality of overlapping subregions of the target on the same strand;
circularizing the hybridized MIPs;
linearizing the circularized MIPs;
amplifying the circularized MIPs to generate amplicons; and
sequencing the amplicons.
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Abstract
The invention generally relates to methods of performing a capture reaction. In certain embodiments, the method involves obtaining a nucleic acid, fragmenting the nucleic acid, and capturing a target sequence on the nucleic acid fragment using a capture moiety, such as a molecular inversion probe.
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10 Claims
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1. A method of improving performance of molecular inversion probe capture reactions, the method comprising:
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obtaining a nucleic acid; fragmenting the nucleic acid into a plurality of nucleic acid fragments to make a target available for capture; introducing a plurality of molecular inversion probes (MIPs) to the plurality of nucleic acid fragments, wherein each of the plurality of MIPs hybridize to the target and captures a different one of a plurality of overlapping subregions of the target on the same strand; circularizing the hybridized MIPs; linearizing the circularized MIPs; amplifying the circularized MIPs to generate amplicons; and sequencing the amplicons. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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Specification