DNA sequencing by synthesis using Raman and infrared spectroscopy detection

• US 10,689,412 B2
• Filed: 04/18/2017
• Issued: 06/23/2020
• Est. Priority Date: 05/23/2011
• Status: Active Grant

• Current Assignee: Trustees Of Columbia University In The City Of New York (Columbia University)
• Original Assignee: Trustees Of Columbia University In The City Of New York (Columbia University)
• Inventors: Ju, Jingyue, Wu, Jian, Li, Zengmin
• Primary Examiner(s): Riley, Jezia
• Application Number:
  US15/490,823
• Publication Number:
  US 20170283451A1
• Time in Patent Office: 1,162 Days
• Field of Search: 536 41, 435 61
• CPC Class Codes: C07H 19/06 Pyrimidine radicals
• CPC Class Codes: C07H 19/16 Purine radicals
• CPC Class Codes: C07H 21/00 Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
• CPC Class Codes: C12Q 1/6827 for detection of mutation or polymorphism
• CPC Class Codes: C12Q 1/6869 Methods for sequencing
• CPC Class Codes: C12Q 1/6874 involving nucleic acid arrays, e.g. sequencing by hybridisation
• CPC Class Codes: C12Q 2525/101 incorporating non-naturally occurring nucleotides, e.g. inosine
• CPC Class Codes: C12Q 2533/101 Primer extension
• CPC Class Codes: C12Q 2565/632 being a surface enhanced, e.g. resonance, Raman spectrometer

Abstract

This invention provides a process of labeling a polynucleotide analogue to be detected by Raman and/or infrared spectroscopy detection.

Claims

1. A nucleoside triphosphate analogue having the structure:

2. The nucleoside triphosphate analogue of claim 1 having a Raman spectroscopy peak with wavenumber from 2100 cm⁻

1 to 2260 cm<sup>−

1</sup>.

3. The nucleoside triphosphate analogue of claim 1, wherein R′

has the structure;

4. The nucleoside triphosphate analogue of claim 3, wherein R′

has the structure;

5. The nucleoside triphosphate analogue of claim 3, wherein R′

has the structure;

6. The nucleoside triphosphate analogue of claim 3, wherein R′

has the structure;

7. The nucleoside triphosphate analogue of claim 3, wherein R″

is —

H.

8. The nucleoside triphosphate analogue of claim 3, wherein R″

is —

OH.

9. The nucleoside triphosphate analogue of claim 3, wherein R′

has a Raman spectroscopy peak with wavenumber at ˜

2105 cm−

1.

10. The nucleoside triphosphate analogue of claim 3, wherein R′

has a Raman spectroscopy peak with wavenumber at ˜

2138 cm−

1.

11. The nucleoside triphosphate analogue of claim 3, wherein R′

has a Raman spectroscopy peak with wavenumber at ˜

2249 cm−

1.

12. The nucleoside triphosphate analogue of claim 3, wherein R′

has a Raman spectroscopy peak with wavenumber at ˜

2259 cm−

1.

13. The nucleoside triphosphate analogue of claim 3, wherein the reducing agent is aqueous Tris-(2-carboxyethyl)phosphine.