Systems and methods for sample preparation, processing and analysis

• US 10,690,627 B2
• Filed: 10/21/2015
• Issued: 06/23/2020
• Est. Priority Date: 10/22/2014
• Status: Active Grant

• Current Assignee: IntegenX Inc. (Thermo Fisher Scientific Incorporated)
• Original Assignee: IntegenX Inc. (Thermo Fisher Scientific Incorporated)
• Inventors: Kindwall, Alexander, Park, Chungsoo Charles, Ilkov, Fedor, Jovanovich, Stevan, King, David, Littlehale, Tristan Robert, Nilson, David Grant, Pittaro, Rick, Schueren, Robert A., Vangbo, Mattias, Wunderle, Philip Justus
• Primary Examiner(s): Martin, Bethany L
• Application Number:
  US14/919,620
• Publication Number:
  US 20160116439A1
• Time in Patent Office: 1,707 Days
• CPC Class Codes: B01L 2200/04 Exchange or ejection of cartridges, containers or reservoirs
• CPC Class Codes: B01L 2200/10 Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2200/16 Reagents, handling or storing thereof
• CPC Class Codes: B01L 2300/0681 Filter
• CPC Class Codes: B01L 2300/0867 Multiple inlets and one sample wells, e.g. mixing, dilution
• CPC Class Codes: B01L 2300/1822 using Peltier elements
• CPC Class Codes: B01L 2300/1827 using resistive heater
• CPC Class Codes: B01L 2400/0421 electrophoretic flow
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: G01N 27/44704 Details; Accessories
• CPC Class Codes: G01N 27/44743 Introducing samples
• CPC Class Codes: G01N 27/44786 of the magneto-electrophoresis type
• CPC Class Codes: G01N 27/44791 Microapparatus sample containers with integrated microfluidic structures B01L3/5027
• CPC Class Codes: G01N 27/453 Cells therefor

Abstract

The present disclosure provides systems and methods for sample preparation, processing and analysis. Also provided in the present disclosure is a fully-integrated electrophoresis cartridge which has a small footprint and configured to removably engage with the system.

Claims

1. An electrophoresis cartridge adapted to releasably engage with a cartridge interface of a system, wherein the electrophoresis cartridge comprises:

(a) an electrophoresis assembly including;

(1) an anode sub-assembly comprising an anode,

(2) a cathode sub-assembly comprising a cathode, and

(3) an electrophoresis capillary having a first end and a second end, wherein said cathode and said anode are configured to provide a voltage gradient across said first end and said second end of said electrophoresis capillary;

(b) a reagent container comprising electrophoresis buffer in fluidic communication with the cathode sub-assembly via a fluid conduit that connects the cathode sub-assembly to the reagent container and is disposed between the electrophoresis capillary and the reagent container;

(c) a pump for pumping electrophoresis buffer from the reagent container through the fluid conduit to the cathode sub-assembly, wherein the cathode sub-assembly, the reagent container, and the pump are in fixed positions on the electrophoresis cartridge; and

(d) a sample inlet port fluidically connected to the cathode sub-assembly.

2. The electrophoresis cartridge of claim 1, further comprising an electrophoresis separation medium container for holding an electrophoresis separation medium, wherein said electrophoresis separation medium container is in fluidic communication with said anode.

3. The electrophoresis cartridge of claim 1, wherein said electrophoresis cartridge is engageable with said cartridge interface to place the sample inlet port of said electrophoresis cartridge in fluid communication with a sample outlet port of said system.

4. The electrophoresis cartridge of claim 2, further comprising a fluid handling device configured to move said electrophoresis separation medium into said electrophoresis capillary.

5. The electrophoresis cartridge of claim 1, further comprising a source of capillary regeneration fluid in communication with said anode sub-assembly.

6. The electrophoresis cartridge of claim 1, further comprising (e) a detection window that exposes at least a portion of said electrophoresis capillary.

7. The electrophoresis cartridge of claim 1, further comprising (e) an electrical interface communicating with said anode and said cathode.

8. The electrophoresis cartridge of claim 1, further comprising a first waste container in fluidic communication with said cathode sub-assembly.

9. The electrophoresis cartridge of claim 8, further comprising a second waste container in fluidic communication with said anode sub-assembly.

10. The electrophoresis cartridge of claim 1, further comprising an outlet and a container containing lysis buffer in fluidic communication with the outlet.

11. The electrophoresis cartridge of claim 1, further comprising an outlet and a container containing water in fluidic communication with the outlet.

12. The electrophoresis cartridge of claim 1, wherein said electrophoresis assembly is a single piece.

13. The electrophoresis cartridge of claim 6, wherein engagement of the electrophoresis cartridge with the cartridge interface of the system automatically establishes an optical communication between a light source in the system and the electrophoresis capillary through the detection window.

14. The electrophoresis cartridge of claim 7, wherein engagement of the electrophoresis cartridge with the cartridge interface of the system automatically establishes an electrical communication between the anode and cathode and a power source in the system through the electrical interface.

15. The electrophoresis cartridge of claim 10, wherein engagement of the electrophoresis cartridge with the cartridge interface of the system automatically establishes a fluidic communication between the container containing lysis buffer and a fluid line of the system through the outlet.

16. The electrophoresis cartridge of claim 11, wherein engagement of the electrophoresis cartridge with the cartridge interface of the system automatically establishes a fluidic communication between the container containing water and a fluid line of the system through the outlet.

17. The electrophoresis cartridge of claim 2, wherein the electrophoresis separation medium container is contained in a cartridge that is removably insertable into the electrophoresis cartridge.

18. An electrophoresis cartridge comprising:

(a) a cartridge housing

(b) an electrophoresis assembly including;

(1) an anode sub-assembly comprising an anode,

(2) a cathode sub-assembly comprising a cathode, and

(3) an electrophoresis capillary having a first end and a second end, wherein said cathode and said anode are configured to provide a voltage gradient across said first end and said second end of said electrophoresis capillary;

(c) a reagent container comprising electrophoresis buffer in fluidic communication with the cathode sub-assembly;

(d) a pump for pumping electrophoresis buffer from the reagent container to the cathode sub-assembly; and

(e) a sample inlet port fluidically connected to the cathode sub-assembly, wherein the cathode sub-assembly, the reagent container, the pump, and the sample inlet port are within the cartridge housing.

19. An electrophoresis cartridge adapted to releasably engage with a cartridge interface of a system, wherein the electrophoresis cartridge comprises:

(a) an electrophoresis assembly including;

(1) an anode sub-assembly comprising an anode,

(2) a cathode sub-assembly comprising a cathode, and

(3) an electrophoresis capillary having a first end and a second end, said cathode and said anode configured to provide a voltage gradient across said first end and said second end of said electrophoresis capillary;

(b) a reagent container comprising electrophoresis buffer;

(c) a pump for pumping electrophoresis buffer from the reagent container to the cathode sub-assembly, the cathode sub-assembly fluidly disposed between the pump and the anode sub-assembly; and

(d) a sample inlet port fluidically connected to the cathode sub-assembly;

wherein the cathode sub-assembly, the reagent container, and the pump are in fixed positions on the electrophoresis cartridge.