Treating diabetes with genetically modified beta cells

• US 10,696,950 B2
• Filed: 03/29/2019
• Issued: 06/30/2020
• Est. Priority Date: 10/03/2017
• Status: Active Grant

• Current Assignee: SDF Biopharma Incorporated
• Original Assignee: Wallkill BioPharma, Inc.
• Inventors: Swiss, Gerald F., Kiewlich, David
• Primary Examiner(s): Hill, Kevin K
• Application Number:
  US16/370,606
• Publication Number:
  US 20190218518A1
• Time in Patent Office: 459 Days
• CPC Class Codes: A61K 35/17 Lymphocytes; B-cells; T-cells; Natural killer cells; Interferon-activated or cytokine-activated lymphocytes when activated by a specific antigen A61K39/00
• CPC Class Codes: A61K 35/39 Pancreas; Islets of Langerhans Langerhans cells of epidermis A61K35/36
• CPC Class Codes: A61P 3/10 for hyperglycaemia, e.g. antidiabetics
• CPC Class Codes: C07K 14/522 Alpha-chemokines, e.g. NAP-2, ENA-78, GRO-alpha/MGSA/NAP-3, GRO-beta/MIP-2alpha, GRO-gamma/MIP-2beta, IP-10, GCP-2, MIG, PBSF, PF-4, KC
• CPC Class Codes: C12N 2501/998 Proteins not provided for elsewhere
• CPC Class Codes: C12N 2502/11 blood or immune system cells
• CPC Class Codes: C12N 2502/99 genetically modified cells
• CPC Class Codes: C12N 2510/00 Genetically modified cells
• CPC Class Codes: C12N 5/0635 B lymphocytes
• CPC Class Codes: C12N 5/0638 Cytotoxic T lymphocytes [CTL] or lymphokine activated killer cells [LAK]
• CPC Class Codes: C12N 5/0646 Natural killers cells [NK], NKT cells
• CPC Class Codes: C12N 5/0676 Pancreatic cells
• CPC Class Codes: C12N 5/0686 Kidney cells

Abstract

Described herein are human transgenic beta cells expressing fugetactic levels of CXCL12 to a subject in need thereof. Also described herein are beta cells comprising a transgene comprising a nucleic acid sequence encoding CXCL12.

Claims

1. A population of isolated genetically engineered human beta cells comprising a nucleic acid encoding for exogenous or heterologous expression of a human CXCL12 protein beta isoform such that said population of said cells is characterized as being resistant to cell death when in the presence of human immune cells in vitro as compared to a population of unengineered human beta cells obtained from a human stem cell that has been differentiated ex vivo into human beta cells,

wherein said characterization is based on the relative amounts of lipase dehydrogenase (LDH) measured after assaying said population of engineered cells for 24 hours in the presence of human peripheral blood mononuclear cells (PBMCs) at a ratio of 1:

30 genetically engineered beta cells to PBMCs,

wherein the amount of LDH is decreased by at least 95% for said population of engineered human beta cells compared to that of a population of unengineered human beta cells,

wherein the nucleic acid is operably linked to an exogenous promoter,

wherein the population of genetically engineered human beta cells is insulin producing and has been exposed to a senescence inducing agent, and

wherein the population of genetically engineered human beta cells is obtained from a human stem cell that has been differentiated ex vivo into a human beta cell and said population of genetically engineered human beta cells is allogeneic or autologous.

2. The population of isolated genetically engineered human beta cells of claim 1, wherein the human CXCL12 protein isoform comprises SEQ ID NO:

2.

3. The population of isolated genetically engineered human beta cells of claim 2,

wherein said population of isolated genetically engineered human beta cells is an induced pluripotent stem (iPS) cell that is an allogeneic iPS differentiated into a human beta cell.

4. The population of claim 1, wherein said characterization is based on the relative amounts of LDH measured after assaying said population of engineered cells for 48 hours in the presence of PMBCs.

5. The population of claim 1, wherein the nucleic acid is heterologous.

6. The population of claim 1, wherein said population of genetically engineered human beta cells is autologous.

7. The population of claim 1, wherein said population of genetically engineered human beta cells is allogeneic.