Ruminant compositions

• US 10,701,955 B2
• Filed: 10/17/2019
• Issued: 07/07/2020
• Est. Priority Date: 01/07/2016
• Status: Active Grant

• Current Assignee: Native Microbials Incorporated
• Original Assignee: Ascus Biosciences, Inc.
• Inventors: Embree, Mallory, Picking, Luke, Gogul, Grant, Tarasova, Janna
• Primary Examiner(s): Paguio Frising, Michelle F.
• Application Number:
  US16/655,776
• Publication Number:
  US 20200037633A1
• Time in Patent Office: 264 Days
• Field of Search: None
• CPC Class Codes: A23K 10/18 of live microorganisms
• CPC Class Codes: A23K 10/30 from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms obtained by microbiological or biochemical processes, e.g. using yeasts or enzymes, A23K10/10
• CPC Class Codes: A23K 10/37 from waste material from hydrolysates of wood or straw A23K10/32; from molasses A23K10/33
• CPC Class Codes: A23K 20/158 Fatty acids; Fats; Products containing oils or fats
• CPC Class Codes: A23K 20/163 Sugars; Polysaccharides
• CPC Class Codes: A23K 20/28 Silicates, e.g. perlites, zeolites or bentonites
• CPC Class Codes: A23K 40/35 Making capsules specially adapted for ruminants
• CPC Class Codes: A23K 50/10 for ruminants
• CPC Class Codes: A23L 31/10 Yeasts or derivatives thereof
• CPC Class Codes: A23L 33/135 Bacteria or derivatives thereof, e.g. probiotics
• CPC Class Codes: A61K 35/74 Bacteria therapeutic use of a bacterial protein A61K38/00
• CPC Class Codes: A61K 35/741 Probiotics probiotic yeast, e.g. saccharomyces A61K36/06
• CPC Class Codes: A61K 35/742 Spore-forming bacteria, e.g. Bacillus coagulans, Bacillus subtilis, clostridium or Lactobacillus sporogenes
• CPC Class Codes: A61K 35/748 Cyanobacteria, i.e. blue-green bacteria or blue-green algae, e.g. spirulina algae, microalgae or microphytes A61K36/02
• CPC Class Codes: A61K 36/06 Fungi, e.g. yeasts
• CPC Class Codes: A61K 36/062 Ascomycota
• CPC Class Codes: A61K 36/064 Saccharomycetales, e.g. baker's yeast
• CPC Class Codes: A61K 39/02 Bacterial antigens
• CPC Class Codes: A61K 9/0019 Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner non-active ingredients are additionally classified in A61K47/00
• CPC Class Codes: A61K 9/0053 Mouth and digestive tract, i.e. intraoral and peroral administration rectal administration A61K9/0031
• CPC Class Codes: A61P 15/04 : for inducing labour or abortion; Uterotonics
• CPC Class Codes: A61P 31/04 : Antibacterial agents
• CPC Class Codes: C12N 1/14 : Fungi culture of mushrooms A01G18/00; as new plants A01H15/00; Culture media therefor
• CPC Class Codes: C12N 1/20 : Bacteria; Culture media therefor

Abstract

The disclosure relates to compositions beneficial for ruminant administration. Particularly, the disclosure provides compositions formulated for ruminants that comprise beneficial microbes.

Claims

1. A powder composition formulated for oral administration to ruminants, comprising:

a) a population of Clostridium comprising bacteria with a 16S nucleic acid sequence sharing at least 99% sequence identity to SEQ ID NO;

28; and

b) a carrier suitable for oral ruminant administration,

wherein the composition is formulated to protect the population of Clostridium from external stressors prior to entering the gastrointestinal tract of the ruminant.

2. The composition of claim 1, wherein the population of Clostridium comprise bacteria with a 16S nucleic acid sequence comprising SEQ ID NO:

28.

3. The composition of claim 1, wherein the composition is formulated to protect the Clostridium from moisture.

4. The composition of claim 1, wherein the powder is dry.

5. The composition of claim 1, wherein the composition is formulated for oral administration to a cow.

6. The composition of claim 1, wherein the composition is further formulated as a solid, liquid, or a mixture thereof.

7. The composition of claim 1, wherein the composition is further formulated in the form of a pellet, capsule, granulate, powder, liquid, or semi-liquid.

8. The composition of claim 1, wherein the composition is combined with a ruminant food.

9. The composition of claim 1, wherein the composition is combined with hay, haylage, silage, livestock feed, forage, fodder, beans, grains, micro-ingredients, fermentation compositions, mixed ration, or a mixture thereof.

10. The composition of claim 1, wherein the composition is combined with total mixed ration.

11. The composition of claim 1, wherein the Clostridium are present in the composition in an amount effective to increase milk production or improve milk compositional characteristics in a ruminant administered the composition, as compared to a ruminant not administered the composition.

12. The composition of claim 1, wherein the population of Clostridium comprise bacteria deposited as NRRL B-67248 or progeny thereof.

13. The composition of claim 1, wherein the composition is stable under ambient conditions for at least one week.

14. The composition of claim 1, wherein the Clostridium are present in the composition in spore form.

15. The composition of claim 1, wherein the Clostridium are present in the composition in an amount of at least 10² cells per gram.