Acoustic perfusion devices
First Claim
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1. A process for separating biomolecules from a fluid mixture, the process comprising:
- flowing a fluid mixture containing biomolecules and cells through an acoustic perfusion device, the acoustic perfusion device comprising;
an acoustic chamber that receives the fluid mixture containing the biomolecules and cells; and
an ultrasonic transducer that includes a piezoelectric material that can be excited to generate a multi-dimensional acoustic standing wave in the acoustic chamber;
exciting the ultrasonic transducer to create the multi-dimensional acoustic standing wave; and
retaining cells with a larger size in the fluid mixture with the multi-dimensional acoustic standing wave.
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Abstract
Acoustic perfusion devices and processes for separating biomolecules from other material in a fluid mixture are disclosed. The devices include an inlet port, an outlet port, and a collection port that are connected to an acoustic chamber. An ultrasonic transducer creates an acoustic standing wave in the acoustic chamber that permits a continuous flow of fluid to be recovered through the collection port while keeping the biological cells within the acoustic chamber to be returned to the bioreactor from which the fluid mixture is being drawn.
746 Citations
17 Claims
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1. A process for separating biomolecules from a fluid mixture, the process comprising:
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flowing a fluid mixture containing biomolecules and cells through an acoustic perfusion device, the acoustic perfusion device comprising; an acoustic chamber that receives the fluid mixture containing the biomolecules and cells; and an ultrasonic transducer that includes a piezoelectric material that can be excited to generate a multi-dimensional acoustic standing wave in the acoustic chamber; exciting the ultrasonic transducer to create the multi-dimensional acoustic standing wave; and retaining cells with a larger size in the fluid mixture with the multi-dimensional acoustic standing wave. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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15. A process for collecting biomolecules from a cell culture, the process comprising:
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flowing a nutrient fluid stream through the cell culture to collect the biomolecules; flowing the nutrient fluid stream through an acoustic perfusion device, the acoustic perfusion device including at least one ultrasonic transducer including a piezoelectric material configured to generate a multi-dimensional acoustic standing wave that holds the cell culture in the acoustic perfusion device; and driving the ultrasonic transducer to generate the multi-dimensional acoustic standing wave and separate any biomolecule aggregates present in the nutrient fluid stream; and separating the biomolecules from the nutrient fluid stream. - View Dependent Claims (16, 17)
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Specification