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Methods of nucleic acid sample preparation

  • US 10,704,082 B2
  • Filed: 09/15/2017
  • Issued: 07/07/2020
  • Est. Priority Date: 09/15/2016
  • Status: Active Grant
First Claim
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1. A method of preparing nucleic acids for analysis, the method comprising:

  • (a) adding one or more nucleotides to a 3′

    end of a double-stranded nucleic acid comprising a target nucleotide sequence, wherein at least one of the one or more nucleotides is a capture moiety modified nucleotide;

    (b) ligating an adapter nucleic acid to the double-stranded nucleic acid to which the capture moiety modified nucleotide has been added to produce a ligation product, wherein a sequence of one or more nucleotides at a 3′

    end of the adapter nucleic acid is complementary with the one or more nucleotides added to the 3′

    end of the double-stranded nucleic acid in step (a);

    (c) capturing the ligation product by contacting the ligation product with a binding partner of the capture moiety modified nucleotide; and

    (d) amplifying the ligation product by polymerase chain reaction using a first target-specific primer that specifically anneals to the target nucleotide sequence and a first adapter primer that specifically anneals to a complementary sequence of the adapter nucleic acid.

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