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Compartmentalised screening by microfluidic control

  • US 10,705,078 B2
  • Filed: 06/26/2019
  • Issued: 07/07/2020
  • Est. Priority Date: 10/12/2004
  • Status: Active Grant
First Claim
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1. A method for screening a repertoire of compounds for a compound having a desired activity, comprising the steps of:

  • (a) providing an aqueous fluid comprising the repertoire of compounds;

    (b) compartmentalizing the repertoire of compounds into microcapsules by partitioning the aqueous fluid with an immiscible fluid as the aqueous fluid is flowing through a microfluidic channel, such that only one compound from the repertoire of compounds is represented in any one microcapsule, and further wherein each microcapsule comprises a target cell; and

    (c) determining an effect of one or more of the repertoire of compounds on the target cell by using a cell-based assay that produces a different reaction product in each microcapsule comprising the target cell and one compound from the repertoire of compounds, wherein a specific reaction product depends on the identity of the compound compartmentalized in each respective microcapsule.

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