×

Preparation of templates for nucleic acid sequencing

  • US 10,710,046 B2
  • Filed: 06/18/2018
  • Issued: 07/14/2020
  • Est. Priority Date: 07/20/2005
  • Status: Active Grant
First Claim
Patent Images

1. A method comprising,(i) amplifying a template using amplification primers on a solid support to produce a plurality of double-stranded nucleic acid molecules such that both strands of each double-stranded nucleic acid molecule are attached to the solid support at their 5′

  • ends, wherein a subset of the amplification primers comprise a cleavage site and wherein the cleavage site is positioned in a double-stranded region of each double-stranded molecule; and

    (ii) cleaving only one strand of the double stranded molecules at the cleavage site to produce a cleaved strand and an un-cleaved strand.

View all claims
  • 3 Assignments
Timeline View
Assignment View
    ×
    ×