×

Midbrain dopamine (DA) neurons for engraftment

  • US 10,711,243 B2
  • Filed: 03/14/2019
  • Issued: 07/14/2020
  • Est. Priority Date: 11/04/2011
  • Status: Active Grant
First Claim
Patent Images

1. An in vitro method for differentiating pluripotent stem cells, comprising:

  • exposing a plurality of pluripotent stem cells to at least one inhibitor of transforming growth factor beta (TGFβ

    )/Activin-Nodal signaling and at least one inhibitor of bone morphogenetic protein (BMP) signaling, and exposing the cells to at least one activator of Sonic hedgehog (SHH) signaling and at least one glycogen synthesis kinase 3β

    (GSK3β

    ) inhibitor that activates wingless (Wnt) signaling, wherein the cells are exposed to the at least one GSK3β

    inhibitor three (3) days from the initial exposure of the cells to the at least one inhibitor of TGFβ

    /Activin-Nodal signaling and the at least one inhibitor of BMP signaling to obtain a cell population comprising greater than about 40% differentiated cells expressing both forkhead box protein A2 (FOXA2) and LIM homeobox transcription factor 1 alpha (LMX1A), and wherein the at least one activator of SHH signaling is selected from the group consisting of a Smoothened agonist (SAG), a modified N-terminal SHH, and a SHH protein comprising a N-terminal fragment and a C-terminal fragment.

View all claims
  • 0 Assignments
Timeline View
Assignment View
    ×
    ×