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Methods and compositions for tagging and analyzing samples

  • US 10,722,858 B2
  • Filed: 07/23/2018
  • Issued: 07/28/2020
  • Est. Priority Date: 03/15/2013
  • Status: Active Grant
First Claim
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1. A method of tagging target oligonucleotides comprising:

  • performing an in vitro transcription reaction on DNA molecules partitioned within a plurality of separate compartments, wherein each DNA molecule comprises an RNA polymerase promoter region, a randomer tag region, and a hybridization region capable of hybridizing to one or more target oligonucleotides, thereby obtaining compartments comprising RNA comprising the RNA polymerase promoter region, the randomer tag region and the hybridization region;

    merging the interior of the compartments comprising RNA with the interior of a set of compartments comprising target oligonucleotides, wherein prior to the merging, the compartments comprising target oligonucleotides are physically separate from the compartments comprising RNA;

    hybridizing the RNA to the target oligonucleotides; and

    performing a reaction to attach a sequence corresponding to the RNA to the target oligonucleotides, thereby tagging the target oligonucleotides.

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