Hydrophilic coating of fluidic channels
First Claim
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1. A device comprising:
- a flowcell comprising a fluidic channel, an inlet port, and an outlet port,wherein the fluidic channel comprises a ceiling, a first sidewall, and a bottom, wherein the ceiling and the first sidewall form a ceiling-first sidewall edge, and wherein the bottom and the first sidewall form a bottom-first sidewall edge,wherein the ceiling comprises a central portion that is offset from the ceiling-first sidewall edge, thereby forming an offset from the ceiling-first sidewall edge,wherein the central portion is more hydrophilic than the offset,wherein the bottom of the fluidic channel comprises a substrate which comprises a plurality of microwells, andwherein the inlet port and the outlet port are in fluid communication with the flowcell via the fluidic channel.
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Abstract
Disclosed herein are methods, devices, and systems for efficient loading and retrieval of particles. In some embodiments, a fluidic channel of a flowcell comprises a ceiling, a first sidewall, and a bottom, wherein the contact angle of the ceiling is at least 10 degrees smaller than the contact angle of the first sidewall, and wherein the bottom of the fluidic channel comprises a substrate that comprises a plurality of microwells.
299 Citations
30 Claims
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1. A device comprising:
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a flowcell comprising a fluidic channel, an inlet port, and an outlet port, wherein the fluidic channel comprises a ceiling, a first sidewall, and a bottom, wherein the ceiling and the first sidewall form a ceiling-first sidewall edge, and wherein the bottom and the first sidewall form a bottom-first sidewall edge, wherein the ceiling comprises a central portion that is offset from the ceiling-first sidewall edge, thereby forming an offset from the ceiling-first sidewall edge, wherein the central portion is more hydrophilic than the offset, wherein the bottom of the fluidic channel comprises a substrate which comprises a plurality of microwells, and wherein the inlet port and the outlet port are in fluid communication with the flowcell via the fluidic channel. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27)
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28. A method for sample loading, comprising
(a) providing a device that comprises: -
a flowcell comprising a fluidic channel, an inlet port, and an outlet port, wherein the fluidic channel comprises a ceiling a first sidewall, and a bottom, wherein the ceiling and the first sidewall forms a ceiling-first sidewall edge, and wherein the bottom and the first sidewall forms a bottom-first sidewall edge, wherein the ceiling comprises a central portion that is offset from the ceiling-first sidewall edge, thereby forming an offset from the ceiling-first sidewall edge, wherein the central portion is more hydrophilic than the offset, wherein the bottom comprises a substrate which comprises a plurality of microwells, and wherein the inlet port and the outlet port are in fluid communication with the flowcell via the fluidic channel; (b) introducing a gas into the fluidic channel via the inlet port; and (c) introducing a first sample comprising a first plurality of particles into the fluidic channel via the inlet port, whereby at least 25% of the plurality of microwells each contains a single particle of the first plurality of particles. - View Dependent Claims (29, 30)
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Specification