Methods of differentiating stem cells into chondrocytes
First Claim
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1. A method of differentiating pluripotent cells to a population of chondrogenic precursors, comprising:
- a) passaging a population of mechanically-dissected pluripotent cell aggregates (PCA) directly on untreated tissue culture-grade plastic until confluency, at least three times to generate mesenchymal stem cell (MSC)-like cells; and
b) culturing the MSC-like cells in a three-dimensional culture, to generate the population of chondrogenic precursors;
wherein the MSC-like cells are positive for CD90, CD73, and CD105.
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Abstract
Disclosed herein are methods of producing chondrocytes from pluripotent stem cells. The invention further provides methods of regenerating cartilaginous tissue.
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Citations
21 Claims
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1. A method of differentiating pluripotent cells to a population of chondrogenic precursors, comprising:
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a) passaging a population of mechanically-dissected pluripotent cell aggregates (PCA) directly on untreated tissue culture-grade plastic until confluency, at least three times to generate mesenchymal stem cell (MSC)-like cells; and b) culturing the MSC-like cells in a three-dimensional culture, to generate the population of chondrogenic precursors; wherein the MSC-like cells are positive for CD90, CD73, and CD105. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
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Specification