Ultrafiltration membrane, device, bioartificial organ, and related methods

• US 10,730,016 B2
• Filed: 10/27/2017
• Issued: 08/04/2020
• Est. Priority Date: 01/10/2007
• Status: Active Grant

• Current Assignee: Cleveland Clinic Foundation
• Original Assignee: Board of Regents of the University of Michigan (University of Michigan)
• Inventors: Fissell, William H., Roy, Shuvo, Fleischman, Aaron, Goldman, Kenneth G.
• Primary Examiner(s): Bass, Dirk R
• Application Number:
  US15/796,162
• Publication Number:
  US 20180141003A1
• Time in Patent Office: 1,012 Days
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: A61M 1/1623 Disposition or location of membranes relative to fluids
• CPC Class Codes: A61M 1/267 used for pumping
• CPC Class Codes: B01D 2257/70 Organic compounds not provided for in groups B01D2257/00 - B01D2257/602
• CPC Class Codes: B01D 2313/243 Pumps
• CPC Class Codes: B01D 2321/22 Electrical effects
• CPC Class Codes: B01D 2325/028 Microfluidic pore structures
• CPC Class Codes: B01D 61/145 Ultrafiltration
• CPC Class Codes: B01D 61/18 Apparatus therefor
• CPC Class Codes: B01D 61/20 Accessories; Auxiliary operations
• CPC Class Codes: B01D 67/0034 by micromachining techniques, e.g. using masking and etching steps, photolithography
• CPC Class Codes: B01D 67/0062 by micromachining techniques, e.g. using masking and etching steps, photolithography
• CPC Class Codes: B01D 67/0088 Physical treatment with compounds, e.g. swelling, coating or impregnation
• CPC Class Codes: G01N 1/4077 by other techniques involving separation of suspended solids
• CPC Class Codes: G01N 2001/4088 filtration

Abstract

The present invention relates to ultrafiltration. In particular, the present invention provides nanoporous membranes having pores for generating in vitro and in vivo ultrafiltrate, devices and bioartificial organs utilizing such nanoporous membranes, and related methods (e.g., diagnostic methods, research methods, drug screening). The present invention further provides nanoporous membranes configured to avoid protein fouling with, for example, a polyethylene glycol surface coating.

Claims

1. An implantable device comprising:

a) a membrane consisting of a plurality of nanofabricated pores having a width less than 500 nanometers, wherein said plurality of nanofabricated pores have a slit shape, wherein the distance between each of said plurality nanofabricated pores is less than 500 nanometers, wherein said plurality of nanofabricated pores are arranged in a rectangular configuration on said membrane, wherein said rectangular configuration is at least a 2;

1 arrangement of said nanofabricated pores on said membrane, wherein said thickness of said membrane is less than 500 nanometers;

b) a housing containing said membrane; and

c) a fluid delivery passageway with a first end and a second end, said first end positioned outside of said housing, said second end positioned to deliver fluid across said membrane.

2. The device of claim 1, wherein said device further comprises a dialysate.

3. The device of claim 1, wherein said device is configured for ultrafiltration.

4. The device of claim 1, wherein said membrane has a porosity below 20%.

5. The device of claim 1, further comprising one or more selected from the group consisting of:

a) a pump configured to deliver said fluid across said membrane,

b) an actuator configured to decrease protein fouling of said nanofabricated pores,

c) a control component used to control/monitor extracellular fluid volume,

d) a population of cells attached to said membrane, wherein said cells are selected from the group consisting of renal tubule cells, pancreatic cells, hepatic cells, thyroid cells, adrenal cells, parathyroid cells, pituitary cells, hypothalamic cells gonadal cells, prokaryotic cells, duodenal cells, gastric cells, intestinal cells, muscle cells, fibroblast cells, and endothelial cells, and

e) one or more sensors associated with said membrane, wherein said sensor is configured to monitor filtration parameters, wherein said one or more sensors are selected from the group consisting of a pressure sensor configured to monitor transmembrane pressure, a proteins sensor configured to monitor protein leakage/membrane breakdown, an optical blood sensor configured to monitor membrane rupture, and urea sensor configured to monitor urea clearance.

6. The device of claim 1, wherein said membrane has a surface coating selected from the group consisting of polyethylene glycol, oligosaccharide surfactant polymers, heparin, and hyaluronan.

7. The device of claim 1, wherein said housing comprises a coating, said coating being biocompatible for in vivo use.

8. The device of claim 1, wherein said nanofabricated pores are modified with polyethylene glycol.

9. The device of claim 1, wherein the material of said membrane is selected from the group consisting of silicon, polysilicon, silicon carbide, ultrananocrystalline diamond, diamond-like-carbon (DLC), silicon dioxide, PMMA, SU-8, PTFE, titanium, silica, silicon nitride, polytetrafluorethylene, polymethylmethacrylate, polystyrene, and silicone.

10. The device of claim 1, wherein said nanofabricated pores are positioned in the center of said membrane.

11. The device of claim 1, wherein said distance between each of said plurality of nanofabricated pores is less than 100 nanometers.

12. The system device of claim 1, wherein said distance between each of said plurality of nanofabricated pores is less than 50 nanometers.

13. The device of claim 1, wherein said housing has a length and a width, said length of said housing being less than 300 millimeters and said width of said housing being less than 300 millimeters.

14. The device of claim 1, wherein said housing comprises a first inlet and a first outlet.

15. The device of claim 14, wherein said first inlet and said first outlet pass through the housing.

16. The device of claim 14, wherein said first inlet and said first outlet further comprise a conduit.

17. The device of claim 16, wherein said conduit comprises a tubing.

18. The device of claim 17, wherein said tubing is attached to said first inlet and said first outlet by a clamp or threaded connection.

19. The device of claim 14, wherein said first inlet and said first outlet comprise a spheroid, elliptical, or slit shape.

20. The device of claim 14, wherein said housing further comprises a second inlet and a second outlet.

21. A method of creating the implantable device of claim 1 comprising imprinting said plurality of nanofabricated pores onto the surface of a membrane, wherein said nanofabricated pores are positioned in the center of said membrane surface, wherein said imprinting is accomplished with nanolithography.

22. A method of filtering a biological fluid comprising:

a) providing;

i) a biological fluid; and

ii) the implantable device of claim 1;

b) transferring said biological fluid into said first end of said delivery passageway; and

c) passing said fluid across said membrane to generate filtered fluid.