Multi-step synthesis of templated molecules
First Claim
1. A library comprising different complexes, said complexes comprising a first entity and a second entity, whereinthe first entity comprises a double stranded oligonucleotide identifier comprising single oligonucleotide strands covalently linked by a first linker, each single strand containing from 12 to 400 nucleic acid monomers, wherein at least one nucleic acid monomer of each single strand is a deoxyribonucleotide,wherein the second entity comprises a small non-peptide molecule and a second linker, the second linker being distinct from the first linker and covalently linking the first entity to the small-non-peptide molecule of the second entity,wherein the small non-peptide molecule of the second entity can be identified by each and both strands of the double stranded oligonucleotide identifier of the first entity, andwherein the small non-peptide molecule is covalently linked to the double stranded identifier oligonucleotide via an anchorage point located at a terminal region of one of the single strands.
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Abstract
Disclosed is a method for the manufacture of a library of complexes. The complexes comprise templated molecules attached to the template which directed the synthesis thereof. The templated molecules are produced in a step-by-step fashion which provides for a high local concentration of reactive groups involved in the formation of connections between the individual components of the template molecule.
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Citations
98 Claims
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1. A library comprising different complexes, said complexes comprising a first entity and a second entity, wherein
the first entity comprises a double stranded oligonucleotide identifier comprising single oligonucleotide strands covalently linked by a first linker, each single strand containing from 12 to 400 nucleic acid monomers, wherein at least one nucleic acid monomer of each single strand is a deoxyribonucleotide, wherein the second entity comprises a small non-peptide molecule and a second linker, the second linker being distinct from the first linker and covalently linking the first entity to the small-non-peptide molecule of the second entity, wherein the small non-peptide molecule of the second entity can be identified by each and both strands of the double stranded oligonucleotide identifier of the first entity, and wherein the small non-peptide molecule is covalently linked to the double stranded identifier oligonucleotide via an anchorage point located at a terminal region of one of the single strands.
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25. A library comprising different complexes, wherein each complex comprises
i) a first entity comprising a double stranded oligonucleotide identifier comprising single oligonucleotide strands covalently linked by a first linker, ii) a second entity comprising a small non-peptide molecule, and iii) a second linker covalently linking the first entity to the small non-peptide molecule of the second entity, wherein the small non-peptide molecule of the second entity can be identified by each and both of the single strands of the double stranded oligonucleotide identifier of the first entity, wherein the small non-peptide molecule is covalently linked to the double stranded identifier oligonucleotide via an anchorage point located at a terminal region of one of the single strands.
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71. A library comprising different complexes, wherein each complex comprises
i) a first entity comprising a double stranded oligonucleotide identifier comprising single oligonucleotide strands covalently linked by a first linker, wherein the first linker links oligonucleotide strand 3′ - and 5′
ends,ii) a second entity comprising a small non-peptide molecule, and iii) a second linker covalently linking the first entity to the second entity, wherein the small non-peptide molecule of the second entity can be identified by each and both of the single strands of the double stranded oligonucleotide identifier, wherein the first entity is covalently linked to the second entity at or near the 3′
or 5′
end of a single strand of the double stranded oligonucleotide identifier. - View Dependent Claims (72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86)
- and 5′
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87. A library comprising different complexes, wherein each complex comprises
i) a first entity comprising a double stranded oligonucleotide identifier comprising single oligonucleotide strands covalently linked by a terminally located hair-pin loop comprising nucleotides, wherein the hairpin loop covalently links oligonucleotide strand 3′ - and 5′
ends.ii) a second entity comprising a molecule selected from the group consisting of monofunctional, difunctional, trifunctional and oligofunctional open-chain hydrocarbons;
monofunctional, difunctional, trifunctional and oligofunctional non-aromatic carbocycles;
monocyclic, bicyclic, tricyclic and polycyclic hydrocarbons;
bridged polycyclic hydrocarbons;
monofunctional, difunctional, trifunctional, and oligofunctional non-aromatic heterocycles;
monocyclic, bicyclic, tricyclic, and polycyclic heterocycles, bridged polycyclic heterocycles;
monofunctional, difunctional, trifunctional and oligofunctional aromatic carbocycles;
monocyclic, bicyclic, tricyclic, and polycyclic aromatic carbocycles; and
monofunctional, difunctional, trifunctional and oligofunctional aromatic heterocycles,iii) a linker covalently linking the first entity and the second entity, wherein the molecule of the second entity can be identified by each and both of the single strands of the double stranded oligonucleotide identifier, wherein the first entity is covalently linked to the second entity at a terminal location of the first entity. - View Dependent Claims (88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98)
- and 5′
Specification