Engineered polymerases for improved sequencing
First Claim
1. An engineered DNA polymerase comprising a variant of SEQ ID NO:
- 1, the variant being at least 95% identical to SEQ ID NO;
1, the variant comprising amino acid substitutions L408F, Y409F and P410T wherein the engineered DNA polymerase has increased accuracy in pairing nucleotides to template bases as compared to a control polymerase having amino acid sequence of SEQ ID NO;
1.
3 Assignments
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Accused Products
Abstract
Provided are nucleic acids encoding engineered polymerases comprising at least one modification in a motif A and/or at least one modification in a motif B of the polymerase and engineered polymerases encoded by the nucleic acids. Also provided are engineered DNA polymerases comprising a variant of SEQ ID NO:1, SEQ ID NO:2, or SEQ ID NO:3, the variant being at least 80% identical to SEQ ID NO:1, SEQ ID NO:2 or SEQ ID NO:3 and comprising an amino acid substitution at one or more positions selected from the group consisting of L408, Y409, P410, R484, A/L485, and I486. Methods, vectors, kits, and compositions comprising the nucleic acids and compositions, methods and kits comprising the engineered polymerases are also provided.
35 Citations
22 Claims
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1. An engineered DNA polymerase comprising a variant of SEQ ID NO:
- 1, the variant being at least 95% identical to SEQ ID NO;
1, the variant comprising amino acid substitutions L408F, Y409F and P410T wherein the engineered DNA polymerase has increased accuracy in pairing nucleotides to template bases as compared to a control polymerase having amino acid sequence of SEQ ID NO;
1. - View Dependent Claims (3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
- 1, the variant being at least 95% identical to SEQ ID NO;
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2. An engineered DNA polymerase comprising a variant of SEQ ID NO:
- 1, the variant being at least 95% identical to SEQ ID NO;
1, the variant comprising amino acid amino acid substitutions L408F, Y409H, and P410V, wherein the engineered DNA polymerase has increased accuracy in pairing nucleotides to template bases as compared to a control polymerase having amino acid sequence of SEQ ID NO;
1. - View Dependent Claims (13, 14, 15, 16, 17, 18, 19, 20, 21, 22)
- 1, the variant being at least 95% identical to SEQ ID NO;
Specification