Compositions containing identifier sequences on solid supports for nucleic acid sequence analysis
First Claim
1. A collection of solid supports comprising single-stranded nucleic acid species,(a) wherein each solid support in the collection comprises more than one single-stranded nucleic acid species covalently linked to the solid support,wherein each single-stranded nucleic acid species linked to a common solid support comprises an identifier sequence and a probe sequence, andwherein the identifier sequence is oriented 5′
- to the probe sequence;
(b) wherein all the single-stranded nucleic acid species linked to the common solid support share the same identifier sequence, andwherein the identifier sequence from the more than one single-stranded nucleic acid species linked to the common solid support in the collection is different from the identifier sequences of the single-stranded nucleic acid species linked to the other solid supports in the collection, whereby each solid support in the collection is associated with a unique identifier sequence; and
(c) wherein the probe sequence of each of the more than one single-stranded nucleic acid species linked to the solid support allows the more than one single-stranded nucleic acid to hybridize a subsequence in a sample nucleic acid, and(d) wherein the probe sequences of the more than one single-stranded nucleic acid species on each solid support comprise at least 10 different species.
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Abstract
Improved solid supports and methods for analyzing target nucleotide sequences are provided herein. Certain improvements are directed to efficiently preparing nucleic acids that comprise nucleotide sequences identical to or substantially identical to one or more target nucleotide sequences, or complement thereof. The prepared nucleic acids include a reference sequence that facilitates sequence analysis. The solid supports and methods provided herein minimize the number of steps required by published sequence analysis methodologies, and thereby offer improved sequence analysis efficiency.
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Citations
27 Claims
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1. A collection of solid supports comprising single-stranded nucleic acid species,
(a) wherein each solid support in the collection comprises more than one single-stranded nucleic acid species covalently linked to the solid support, wherein each single-stranded nucleic acid species linked to a common solid support comprises an identifier sequence and a probe sequence, and wherein the identifier sequence is oriented 5′ - to the probe sequence;
(b) wherein all the single-stranded nucleic acid species linked to the common solid support share the same identifier sequence, and wherein the identifier sequence from the more than one single-stranded nucleic acid species linked to the common solid support in the collection is different from the identifier sequences of the single-stranded nucleic acid species linked to the other solid supports in the collection, whereby each solid support in the collection is associated with a unique identifier sequence; and (c) wherein the probe sequence of each of the more than one single-stranded nucleic acid species linked to the solid support allows the more than one single-stranded nucleic acid to hybridize a subsequence in a sample nucleic acid, and (d) wherein the probe sequences of the more than one single-stranded nucleic acid species on each solid support comprise at least 10 different species. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27)
- to the probe sequence;
Specification