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Method for detecting multiple predetermined compounds in a sample

  • US 10,732,183 B2
  • Filed: 03/14/2014
  • Issued: 08/04/2020
  • Est. Priority Date: 03/15/2013
  • Status: Active Grant
First Claim
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1. A method for detecting interaction of at least two predetermined compounds which comprises:

  • (a) contacting at least a first predetermined compound present in a sample with a detectable tag moiety, wherein the detectable tag moiety comprises at least one detectable component, under conditions permitting the tag moiety to be conjugated to the predetermined compound present in the sample to form a detectably tagged predetermined compound;

    (b) treating the sample from step (a) to form an electrolyte solution containing the detectably tagged predetermined compounds;

    (c) contacting the electrolyte solution from step (b) with at least one nanopore, wherein at least a second predetermined compound is immobilized on the top of said at least one nanopore, under conditions permitting the binding of the detectably tagged predetermined compound with the second predetermined compound;

    (d) treating the detectably tagged predetermined compound bound to said at least one nanopore so as to separate the tag moiety from the predetermined compound; and

    (e) detecting said at least one detectable component of the tag moiety so released as the tag moiety passes through said at least one nanopore so as to thereby detect the interaction of the predetermined compounds.

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