Aromatic amino acid catabolism enzymes

• US 20010005749A1
• Filed: 12/21/2000
• Published: 06/28/2001
• Est. Priority Date: 07/31/1998
• Status: Abandoned Application

• Current Assignee: Anthony J. Kinney, Edgar B. Cahoon, Michele Morgante, Rafalski J. Antoni, Rebecca E. Cahoon, Saverio C. Falco, William D. Hitz
• Original Assignee: Anthony J. Kinney, Edgar B. Cahoon, Michele Morgante, Rafalski J. Antoni, Rebecca E. Cahoon, Saverio C. Falco, William D. Hitz
• Inventors: Hitz, William D., Falco, Saverio Carl, Cahoon, Rebecca E., Kinney, Anthony J., Rafalski, J. Antoni, Morgante, Michele, Cahoon, Edgar B.
• Application Number:
  US09/742,954
• Publication Number:
  US 20010005749A1
• Time in Patent Office: Days
• US Class Current: 536/23.2
• CPC Class Codes: C12N 15/8251 Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
• CPC Class Codes: C12N 9/0069 acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
• CPC Class Codes: C12N 9/14 Hydrolases (3)
• CPC Class Codes: C12P 13/22 Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
• CPC Class Codes: C12Y 113/11005 Homogentisate 1,2-dioxygenase (1.13.11.5)
• CPC Class Codes: C12Y 307/01002 Fumarylacetoacetase (3.7.1.2)

Abstract

This invention relates to an isolated nucleic acid fragment encoding an aromatic amino acid catabolic enzyme. The invention also relates to the construction of a chimeric gene encoding all or a portion of the aromatic amino acid catabolic enzyme, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the aromatic amino acid catabolic enzyme in a transformed host cell.

Claims

1. An isolated nucleic acid fragment encoding a homogentisate 1,2-dioxygenase comprising a member selected from the group consisting of:

(a) an isolated nucleic acid fragment encoding the amino acid sequence set forth in a member selected from the group consisting of SEQ ID NO;

2, SEQ ID NO;

4, SEQ ID NO;

6 and SEQ ID NO;

8;

(b) an isolated nucleic acid fragment that is complementary to (a).

2. The isolated nucleic acid fragment of

claim 1

wherein nucleic acid fragment is a functional RNA.

3. The isolated nucleic acid fragment of

claim 1

wherein the nucleotide sequence of the fragment comprises the sequence set forth in a member selected from the group consisting of SEQ ID NO:

1, SEQ ID NO;

3, SEQ ID NO;

5 and SEQ ID NO;

7

4. A chimeric gene comprising the nucleic acid fragment of

claim 1

operably linked to suitable regulatory sequences.

5. A transformed host cell comprising the chimeric gene of

claim 4

.

6. An isolated nucleic acid fragment encoding a fumarylacetoacetase comprising a member selected from the group consisting of:

(a) an isolated nucleic acid fragment encoding the amino acid sequence set forth in a member selected from the group consisting of SEQ ID NO;

10, SEQ ID NO;

12 and SEQ ID NO;

14;

(b) an isolated nucleic acid fragment that is complementary to (a).

7. The isolated nucleic acid fragment of

claim 6

wherein nucleic acid fragment is a functional RNA.

8. The isolated nucleic acid fragment of

claim 6

wherein the nucleotide sequence of the fragment comprises the sequence set forth in a member selected from the group consisting of SEQ ID NO:

9, SEQ ID NO;

11 and SEQ ID NO;

13.

9. A chimeric gene comprising the nucleic acid fragment of

claim 6

operably linked to suitable regulatory sequences.

10. A transformed host cell comprising the chimeric gene of

claim 9

.