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Electrochemical analyte sensors using thermostable soybean peroxidase

  • US 20010017269A1
  • Filed: 03/23/2001
  • Published: 08/30/2001
  • Est. Priority Date: 10/11/1995
  • Status: Active Grant
First Claim
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1. An electrochemical sensor, comprising:

  • (a) an electrode;

    (b) a hydrogen peroxide sensing layer on the electrode, comprising a thermostable peroxidase and non-leachable redox centers for transferring electrons between the electrode and the peroxidase; and

    (c) a second enzyme capable of catalyzing the production of hydrogen peroxide in the presence of an analyte, the second enzyme being disposed on the electrode proximate the sensing layer but electrically insulated from the sensing layer;

    wherein the sensor operates continuously at 37°

    C. for 100 hours or more with less than 10% drop in the sensor'"'"'s output.

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