Method for selectively isolating a nucleic acid
First Claim
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1. A method for separating a nucleic acid of interest from a population of nucleic acid molecules, the method comprising;
- providing a population of nucleic acid molecules;
contacting said population of nucleic acid molecules with a first targeting element, wherein said first targeting element binds specifically to at least one nucleic acid sequence of interest in said population of nucleic acid molecules;
attaching a separation group to said targeting element;
immobilizing said attached separation group to a substrate, thereby forming an immobilized targeting element-separation group complex; and
removing said immobilized targeting element-separation group complex from said population of nucleic acid molecules, thereby separating said nucleic acid sequence of interest from said population of nucleic acid molecules.
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Abstract
Provided are methods for selectively identifying and isolating nucleic acids in a population of nucleic acid molecules.
28 Citations
20 Claims
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1. A method for separating a nucleic acid of interest from a population of nucleic acid molecules, the method comprising;
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providing a population of nucleic acid molecules;
contacting said population of nucleic acid molecules with a first targeting element, wherein said first targeting element binds specifically to at least one nucleic acid sequence of interest in said population of nucleic acid molecules;
attaching a separation group to said targeting element;
immobilizing said attached separation group to a substrate, thereby forming an immobilized targeting element-separation group complex; and
removing said immobilized targeting element-separation group complex from said population of nucleic acid molecules, thereby separating said nucleic acid sequence of interest from said population of nucleic acid molecules. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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19. A method for separating a nucleic acid of interest from a population of nucleic acid molecules, the method comprising;
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(a) providing a population of nucleic acid molecules;
(b) contacting said population of nucleic acid molecules with a targeting element attached to a separation group, wherein said targeting element binds specifically to at least one nucleic acid sequence of interest in said population of nucleic acid molecules;
(c) removing said separation group from said bound targeting element;
(d) immobilizing said attached separation group to a substrate, thereby forming an immobilized targeting element-separation group complex; and
(e) removing said immobilized targeting element-separation group complex from nucleic acid of interest, thereby separating said nucleic acid sequence of interest from said population of nucleic acid molecules. - View Dependent Claims (20)
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Specification