Cross channel device for serial sample injection
First Claim
1. A microfabricated analytical device for at least partially separating the components of a sample, the analytical device comprising:
- (a) a first channel having a sample reservoir at one end and a waste reservoir at an opposite end;
(b) a second channel which intersects across the first channel, the second channel comprising an electrophoretic separation channel; and
(c) a pressure system adapted to generate a pressure differential across the first channel so as to move a sample from the sample reservoir across the first channel and into an intersection between the first and second channels.
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Abstract
The present invention provides a microfluidic analytical device and accompanying methods for injecting a sample and separating the components therein comprising an injection channel and an intersecting separation channel, the two channels formed to be in continuous fluid communication with one another. The separation channel preferably includes a separation material for resolving the components in a sample. The methods include using a first force, preferably a pressure differential, and a second force, preferably an electric field, to move samples through the microchannels. The device and methods are specially adapted to accommodate multiple sample injection and separation. Thus, the device and methods allow for increased sample throughput and are simple and inexpensive to carry out compared to related devices and methods.
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Citations
41 Claims
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1. A microfabricated analytical device for at least partially separating the components of a sample, the analytical device comprising:
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(a) a first channel having a sample reservoir at one end and a waste reservoir at an opposite end;
(b) a second channel which intersects across the first channel, the second channel comprising an electrophoretic separation channel; and
(c) a pressure system adapted to generate a pressure differential across the first channel so as to move a sample from the sample reservoir across the first channel and into an intersection between the first and second channels. - View Dependent Claims (3, 4, 5, 6, 7, 9, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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2. A microfabricated analytical device for at least partially separating the components of a sample, the analytical device comprising:
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(a) a first channel having a sample reservoir at one end and a waste reservoir at an opposite end;
(b) a second channel which intersects across the first channel, the second channel comprising an electrophoretic separation channel; and
(c) an electrokinetic system adapted to electrokinetically move a sample from the sample reservoir across the first channel and into an intersection between the first and second channels.
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8. An analytical device for at least partially separating the components of a sample, the analytical device comprising:
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(a) a sample reservoir;
(b) a first channel extending from the sample reservoir;
(c) a second channel which intersects with the first channel, the first and second channels being in fluid communication;
(d) a pressure differential generator adapted to generate a pressure differential across the first channel; and
(e) an electric field generator adapted to create an electrical field in the second channel. - View Dependent Claims (10)
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20. A method for transporting a sample using a device which includes a first channel having a sample reservoir at one end and a waste reservoir at an opposite end and a second channel which intersects across the first channel, comprising:
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(a) loading the sample into the sample reservoir;
(b) generating a first force in the first channel to move the sample along the first channel; and
(c) applying a second force in the second channel to move at least a portion of the sample into the second channel, the second force being of a different type than the first force. - View Dependent Claims (21, 22, 23, 24, 25, 26, 27, 28)
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29. A method for analyzing a sample using a device which includes (i) a sample reservoir into which the sample is placed;
- (ii) a first channel in fluid communication with the sample reservoir and adapted for receiving the sample, (iii) a second channel which intersects with the first channel and which is adapted to receive at least a portion of the sample, the first and second channel being connected so as to provide continuous fluid communication between the first and second channel, (iv) a pressure differential generator, and (v) an electric field generator, the method comprising;
(a) loading the sample into the sample reservoir;
(b) generating a pressure differential in the first channel with the pressure differential generator, the pressure differential acting to move the sample from the sample reservoir into and along the first channel; and
(c) applying an electric field to the second channel using the electric field generator, the electric field acting to move at least a portion of the sample into the second channel. - View Dependent Claims (30, 31, 32, 33, 34, 35)
- (ii) a first channel in fluid communication with the sample reservoir and adapted for receiving the sample, (iii) a second channel which intersects with the first channel and which is adapted to receive at least a portion of the sample, the first and second channel being connected so as to provide continuous fluid communication between the first and second channel, (iv) a pressure differential generator, and (v) an electric field generator, the method comprising;
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36. An analytical method for the serial injection of multiple samples using a device which includes (i) a sample reservoir designed to receive the samples, (ii) a first channel in fluid communication with the sample reservoir and adapted for receiving the samples, and (ii) a second channel which intersects at an angle with the first channel and is adapted to receive a portion of the samples, the method comprising:
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(a) loading multiple samples into the sample reservoir; and
(b) controllably moving the samples along the first channel and diverting a portion of each of the samples into the second channel. - View Dependent Claims (37, 38, 39, 40, 41)
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Specification