Methods and compositions for amplifying detectable signals in specific binding assays
First Claim
1. A method for detecting a nucleic acid target, the method comprising:
- a) hybridizing a nucleic acid target, comprising a target nucleic acid sequence, to a nucleic acid probe, comprising a probe nucleic acid sequence, wherein the target comprises a binding ligand;
b) contacting the hydridized target with a receptor comprising multiple sites capable of binding the binding ligand to complex the receptor to the binding ligand;
c) contacting the receptor with an amplification reagent, comprising a plurality of the binding ligands, to complex the amplification reagent to the receptor; and
d) detecting the presence of the complexed amplification reagent.
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Abstract
Methods and compounds are provided for detecting target molecules in a sample using specific binding assays. In particular, methods are provided for detecting a nucleic acid target in a sample. In one embodiment, the method comprises hybridizing a nucleic acid target, comprising a target nucleic acid sequence, to a nucleic acid probe, comprising a probe nucleic acid sequence, wherein the target comprises a binding ligand. The hydridized target is contacted with a receptor comprising multiple sites capable of binding the binding ligand to complex the receptor to the binding ligand, and the receptor is contacted with an amplification reagent, comprising a plurality of the binding ligands, to complex the amplification reagent to the receptor. The presence of the complexed amplification reagent then is detected, for example, by detecting the presence of a detectable label, such as a fluorescent label, for example, on the receptor or the amplification reagent. Optionally, the amplification reagent, comprising a plurality of the binding ligands, is contacted with labeled receptor molecules thereby to complex a plurality of labeled receptor molecules to the amplification reagent, and the labeled receptor molecules complexed to the amplification reagent are detected. This permits the detectable signal to be enhanced and amplified. In one embodiment, the binding ligand is biotin, the receptor is streptavidin, and the amplification reagent is an antibody or a DNA matrix. In another embodiment, an array of different nucleic acid probes immobilized on a surface, each having a defined sequence and location on the surface, may be used in the assays, thus permitting screening and detection of binding of a large number of nucleic acids.
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Citations
35 Claims
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1. A method for detecting a nucleic acid target, the method comprising:
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a) hybridizing a nucleic acid target, comprising a target nucleic acid sequence, to a nucleic acid probe, comprising a probe nucleic acid sequence, wherein the target comprises a binding ligand;
b) contacting the hydridized target with a receptor comprising multiple sites capable of binding the binding ligand to complex the receptor to the binding ligand;
c) contacting the receptor with an amplification reagent, comprising a plurality of the binding ligands, to complex the amplification reagent to the receptor; and
d) detecting the presence of the complexed amplification reagent. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22)
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23. A method for detecting a nucleic acid target, the method comprising:
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a) providing a substrate comprising a surface, the surface comprising at least 100 nucleic acid probes, each nucleic acid probe contained in an area of less than about 0.1 cm2, and each nucleic acid probe having a defined sequence and location on the surface;
b) contacting the surface with a nucleic acid target, comprising a target nucleic acid sequence, to permit the nucleic acid target to hybridize with at least one selected nucleic acid probe that comprises a probe nucleic acid sequence capable of hybridizing to the target nucleic acid sequence, and wherein the target comprises a binding ligand;
c) contacting the hydridized target with a receptor comprising multiple sites capable of binding the binding ligand to complex the receptor to the binding ligand;
d) contacting the receptor with an amplification reagent, comprising a plurality of the binding ligands, to complex the amplification reagent to the receptor; and
e) detecting the presence of the complexed amplification reagent. - View Dependent Claims (24, 25, 26, 27)
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28. A complex comprising:
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a nucleic acid comprising a binding ligand;
a receptor comprising multiple binding sites capable of binding the binding ligand; and
an amplification reagent comprising a plurality of said binding ligands. - View Dependent Claims (29, 30, 31)
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32. A substrate comprising a surface having immobilized thereon a nucleic acid probe, comprising a probe nucleic acid sequence, hybridized to a nucleic acid target, comprising a target nucleic acid sequence;
wherein the target comprises a binding ligand, and wherein the binding ligand on the target is complexed with a receptor comprising multiple sites capable of binding the binding ligand, and wherein the receptor is complexed to an amplification reagent, comprising a plurality of the binding ligands. - View Dependent Claims (33, 34, 35)
Specification