In situ bioreactors and methods of use thereof
First Claim
1. An in situ bioreactor adapted for systemic delivery of bioactive agents, comprising a first nucleic acid molecule encoding a cell growth stimulating agent, a second nucleic acid molecule encoding a bioactive agent, and a biocompatible substance capable of cellular infiltration.
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Abstract
The present invention provides in situ bioreactors comprising a biocompatible substance comprising nucleic acid molecules and capable of cellular ingrowth and systemic delivery of a bioactive agent. Also provided are compositions, devices, and kits comprising the same. In various embodiments the biocompatible substance comprises a matrix and at least one nucleic acid molecule encoding a bioactive agent. In other embodiments bioreactors are provided wherein a first gene that encodes a growth factor is present and a second gene encoding a bioactive agent is present during manufacture or provided to the bioreactor following manufacture or implantation.
84 Citations
104 Claims
- 1. An in situ bioreactor adapted for systemic delivery of bioactive agents, comprising a first nucleic acid molecule encoding a cell growth stimulating agent, a second nucleic acid molecule encoding a bioactive agent, and a biocompatible substance capable of cellular infiltration.
- 82. A method adapted for systemic delivery of a protein from a tissue site, comprising introducing into a tissue site of an animal an in situ bioreactor, the bioreactor comprising a first nucleic acid molecule encoding a cell growth stimulating agent, wherein the bioreactor comprises a biocompatible substance capable of infiltration by cells, and wherein a second nucleic acid molecule encoding a serum soluble protein is introduced into the bioreactor following cellular infiltration.
- 83. A method adapted for systemic delivery of a protein from a tissue site, comprising contacting a tissue site of an animal with an in situ bioreactor, wherein the bioreactor comprises a biocompatible substance and a first nucleic acid molecule, wherein the first nucleic acid molecule encodes a cell growth stimulating agent, and wherein said encoded cell growth stimulating agent conditions matrix infiltrating cells for uptake of a second nucleic acid molecule encoding a serum soluble protein.
- 89. A method adapted for systemic delivery of a protein from a tissue site, comprising introducing into a tissue site of an animal an in situ bioreactor, wherein the bioreactor comprises a biocompatible substance, a cell growth stimulating agent, and a first nucleic acid molecule encoding a serum soluble protein wherein the bioreactor is capable of infiltration by cells.
- 98. A Bi-gene device comprising a biocompatible substance capable of cellular infiltration, a first nucleic acid molecule encoding a cell growth stimulating agent, and a second nucleic acid molecule encoding a bioactive agent.
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103. A kit for the production of a device, comprising:
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a) an appropriate container, b) a biocompatible substance, c) a first nucleic acid molecule encoding a cell growth stimulating agent; and
d) a second nucleic acid molecule encoding a bioactive agent.
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104. A kit for the production of a coated device, comprising:
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a) a device coated with a biocompatible substance, b) a first nucleic acid molecule encoding a growth stimulating agent; and
c) a second nucleic acid molecule encoding a bioactive agent.
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Specification