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Methods and apparatus for detecting nucleic acid polymorphisms

  • US 20010046673A1
  • Filed: 01/25/2001
  • Published: 11/29/2001
  • Est. Priority Date: 03/16/1999
  • Status: Abandoned Application
First Claim
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1. A method of detecting an allele at a polymorphic site in a nucleic acid molecule, the method comprising:

  • locating a sample containing a nucleic acid molecule at an examination site;

    contacting the sample with a primer and at least one photoluminescent dideoxy terminator, where the primer binds specifically to a nucleic acid sequence adjacent to a polymorphic site in the nucleic acid molecule;

    modifying the primer so that the polarization of light emitted by the terminator in response to illumination with polarized light is larger upon incorporation of the terminator into the modified primer than upon incorporation of the terminator into an unmodified primer;

    illuminating the sample with polarized light capable of stimulating photoluminescence emission from the terminator;

    detecting polarized light emitted from the sample sequence; and

    determining the identity of an allele at the polymorphic site based on the extent of polarization of the light transmitted from the sample.

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