Devices for performing array hybridization assays and methods of using the same
First Claim
1. An array hybridization chamber for receiving at least one array to be hybridized, comprising:
- a bottom surface;
at least one adjustable spacing element within the chamber to adjust a spacing between the bottom surface and the array between first and second positions in which a film of fluid can and cannot, respectively, be held by surface tension between the array and bottom surface.
1 Assignment
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Accused Products
Abstract
Array hybridization devices and methods for their use are provided. The subject devices are characterized by having a substantially planar bottom surface, a cover, at least one fluid port and at least one adjustable spacing element for adjusting the spacing between an array and the bottom surface. In using the subject devices, an array is placed on the at least one adjustable spacing element in the chamber and the space between the array and the bottom surface is adjusted by moving the at least one adjustable spacing element. The adjusted array is contacted with at least one biological sample introduced into the chamber. The subject inventions find use in a variety of array-based applications, including nucleic acid array hybridizations.
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Citations
34 Claims
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1. An array hybridization chamber for receiving at least one array to be hybridized, comprising:
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a bottom surface;
at least one adjustable spacing element within the chamber to adjust a spacing between the bottom surface and the array between first and second positions in which a film of fluid can and cannot, respectively, be held by surface tension between the array and bottom surface. - View Dependent Claims (2, 32, 33, 34)
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3. An array hybridization chamber comprising:
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a. a bottom surface for receiving at least one array to be hybridized, b. at least one adjustable spacing element for adjusting the spacing between said at least one array and said bottom surface, c. a cover operatively associated with said bottom surface, wherein said cover forms a sealed enclosure with said bottom surface when in a closed position, and d. at least one fluid port for delivering and/or removing fluid from said chamber. - View Dependent Claims (4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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16. A method for contacting a sample to an array, said method comprising:
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(a) contacting the array with said sample when the array is in a first position facing a surface; and
(b) adjusting the spacing between the array and the surface. - View Dependent Claims (17, 18)
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19. A method for contacting a sample to an array, said method comprising:
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a. providing an array hybridization chamber comprising at least one adjustable spacing element, b. placing at least one array onto said at least one adjustable spacing element, c. adjusting said array in said chamber by moving said at least one adjustable spacing element, and d. introducing at least one biological sample into said chamber. - View Dependent Claims (20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31)
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Specification