Cardiac valve replacement
First Claim
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1. A bioprosthetic heart valve comprising an acellular matrix and isolated myofibroblasts wherein at least 60% of the total collagen produced by said myofibroblasts is type I collagen.
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Abstract
The invention provides a replacement heart valve which contains an acellular matrix as a structural scaffold. The scaffold is seeded with isolated myofibroblasts and/or endothelial cells prior to implantation into a recipient mammal.
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Citations
22 Claims
- 1. A bioprosthetic heart valve comprising an acellular matrix and isolated myofibroblasts wherein at least 60% of the total collagen produced by said myofibroblasts is type I collagen.
- 5. A valve comprising an acellular matrix and an isolated myofibroblast, wherein less than 25% of total collagen production by said myofibroblast is type III collagen.
- 11. A method of enhancing production of type I collagen by an isolated myofibroblast, comprising culturing said myofibroblast under pulsatile flow conditions.
- 15. A method of enhancing viability and contractile activity of myofibroblasts in vitro comprising culturing said myofibroblast under pulsatile flow conditions.
- 19. An isolated myofibroblast, wherein said myofibroblast is genetically altered to increase type I collagen production relative to type III collagen production.
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21. A method of manufacturing an artificial heart valve, comprising
(a) providing an acellular matrix, (b) seeding said matrix with isolated myofibroblasts; - and
(c) culturing said myofibroblasts under pulsatile flow conditions. - View Dependent Claims (22)
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Specification