Scanning optical detection system
First Claim
1. A system for optical detection of emitted radiation from microlocations on an object to be examined, comprising:
- a light source providing excitation radiation characterized in that the diameter of the excitation radiation when illuminating the microlocation is of the same diameter or less than the diameter of the microlocation to be examined, a confocal scanning system adapted to receive the excitation radiation and direct it to the microlocation and to provide reflected radiation and emitted radiation from the microlocation, a first detector adapted to receive the reflected radiation and a position detection system, the first detector having an output connected to a position detection system, a second detector operatively positioned to receive the emitted radiation from the microlocation, the detector characterized in that the diameter examined by the detector is less than or equal to the diameter of the excitation radiation, and a control system coupled to the position detection system which causes the confocal scanning system to direct excitation radiation to a specific microlocation.
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Abstract
An optical detection system is adapted particularly for detection of biological reactions, especially fluorescent or chemilluminescent reactions. An excitation source, preferably a laser, illuminates a portion of an object to be examined, the portion preferably comprising one microlocation out of an array of microlocations. An intervening optical detection platform serves to direct the excitation radiation, preferably through use of a scanning system, most preferably through use of an x-y mirror-based scanning system to the portion of the object to be illuminated. A detector, preferably a photomultiplier tube, receives the emitted radiation from the objects to be examined, the detector being characterized in that the diameter of the region examined by the detector is the same as or smaller than the diameter of the illuminated region, and comprises less than the entire surface of the object to be examined, and most preferably images a whole or a part of a single microlocation. Preferably, the excitation source is coupled to the optical detection platform via an optical fiber. In operation, a confocal microscopy system is formed in which the excitation radiation is substantially in focus at the surface of the object to be examined, the excitation radiation having a lateral extent less than the entire diameter of the object to be examined and the detection system having a lateral field of view of a diameter substantially the same as or less than the diameter of the excitation region. In one aspect of this invention, the optical detection platform includes an excitation detector which measures reflected excitation radiation from the object to be examined. This information is then compared to prestored information regarding the location of the microlocations and interstitial regions on the object to be examined, whereby alignment information is obtained. The excitation radiation may then be precisely directed to a given microlocation or portion thereof so as to perform the examining through the confocal system. Significant increases in signal-to-noise ratio are achieve.
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Citations
33 Claims
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1. A system for optical detection of emitted radiation from microlocations on an object to be examined, comprising:
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a light source providing excitation radiation characterized in that the diameter of the excitation radiation when illuminating the microlocation is of the same diameter or less than the diameter of the microlocation to be examined, a confocal scanning system adapted to receive the excitation radiation and direct it to the microlocation and to provide reflected radiation and emitted radiation from the microlocation, a first detector adapted to receive the reflected radiation and a position detection system, the first detector having an output connected to a position detection system, a second detector operatively positioned to receive the emitted radiation from the microlocation, the detector characterized in that the diameter examined by the detector is less than or equal to the diameter of the excitation radiation, and a control system coupled to the position detection system which causes the confocal scanning system to direct excitation radiation to a specific microlocation. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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19. A confocal microscopy system including an optical detection platform comprising:
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a restricted excitation source characterized in that the diameter of the excitation radiation incident upon the object to be examined is less than the lateral dimension of the object to be examined and at least substantially five times greater than the diffraction limited spot size of the excitation source, and a detector characterized in that it has a restricted field of view in diameter which is no larger than the diameter of the excitation radiation incident upon the object to be examined. - View Dependent Claims (20, 21, 22, 23, 24, 25, 26)
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27. A method for optically examining a microlocation on an object comprising the steps of:
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illuminating at least a portion of the object by scanning light from a source through a scanning confocal microscope onto the object, and detecting light reflected from the object through the scanning confocal microscope, determining the position of the microlocation by analyzing the detected reflected light, illuminating a microlocation with light from the source through the scanning confocal microscope utilizing the determined position of the microlocation, and detecting emitted radiation from the microlocation. - View Dependent Claims (28, 29, 30)
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31. A method for examining an object having multiple microlocations separated by interstitial areas comprising the steps of:
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illuminating multiple points on the object to be examined, detecting reflected radiation from the object to be examined, comparing the information constituting reflected radiation with information regarding the structure of the object to be examined, whereby the position of the object is determined, and illuminating one microlocation through a confocal microscope based upon the position information. - View Dependent Claims (32)
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33. A method for determining fluorescence intensity from multiple microlocations disposed on the surface of a biological diagnostic system comprising the steps of:
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scanning the surface of the diagnostic system which includes the microlocations with a laser source directed through a scanning confocal optical system, detecting light reflected from the microlocations, determining the position of the microlocations by imaging the reflected light, illuminating one microlocation through the confocal optical system based upon the determined position, wherein the illumination does not extend substantially beyond the microlocation, and detecting from the microlocation via the confocal microscope, where the detector masks emissions from the object in regions other than the one micro location.
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Specification