Simultaneous imaging and spectroscopy apparatus

• US 20010052979A1
• Filed: 03/07/2001
• Published: 12/20/2001
• Est. Priority Date: 03/07/2000
• Status: Active Grant

• Current Assignee: Chemimage Corporation
• Original Assignee: Chemimage Corporation
• Inventors: Zugates, Christopher T., Bangalore, Arjun, Nelson, Matthew P., Treado, Patrick J.
• Granted Patent: US 6,717,668 B2
• Time in Patent Office: Days
• US Class Current: 356/326
• CPC Class Codes: G01B 11/2545 with one projection direction and several detection directions, e.g. stereo
• CPC Class Codes: G01J 3/2823 Imaging spectrometer
• CPC Class Codes: G01J 3/44 Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
• CPC Class Codes: G01J 3/447 Polarisation spectrometry
• CPC Class Codes: G02B 21/365 Control or image processing arrangements for digital or video microscopes G02B21/361, G02B21/362 take precedence

Abstract

An apparatus incorporated within a spectroscopic imaging system, typically a microscope, but also applicable to other image gathering platforms, namely fiberscopes, macrolens imaging systems and telescopes employing a polarizing beam splitting element is disclosed. The apparatus allows simultaneous spectroscopic (i.e. chemical) imaging and rapid acquisition spectroscopy to be performed without the need for moving mechanical parts or time sequenced sampling and without introducing significant optical signal loss or degradation to the spectroscopic imaging capability. In addition, the apparatus affords a more compact design, an improved angular field of view and an improved overall ruggedness of optical design at a lower manufacturing and maintenance cost compared to previous devices.

Claims

1. A spectroscopic imaging system for enabling simultaneous collection of a spectrum and a spectroscopic image from a sample comprising:

a) a polarization beamsplitter for separating light from said sample into parallel and perpendicular polarized light components;

b) a spectroscopic imaging system for converting the parallel light into a spectroscopic image of said sample; and

c) a spectra generating system for converting the perpendicular light into at least one spectra of said sample.

2. The system of

claim 1

wherein the spectroscopic imaging system comprises an objective for collecting a spectrum of scattered light from said parallel light transmitted by said beamsplitter and producing a collimated beam therefrom;

a filter for selecting a spectroscopic image of said collimated beam and producing a filtered spectroscopic image therefrom; and

a detector for collecting said filtered spectroscopic image.

3. The system of

claim 2

wherein said filter is at least one of a liquid crystal tunable filter, an acousto-optic tunable filter, a Fabry-perot filter, a filter wheel, a Sagnac interferometer, a Michelson interferometer, and a diffraction grating.

4. The system of

claim 1

wherein the spectra generating system comprises an objective for collecting a spectrum of scattered light from said perpendicular light transmitted by said beamsplitter and producing a collimated beam therefrom having a two-dimensional image;

a fiber optic array for producing a one-dimensional array from said two-dimensional image; and

a spectrometer for producing at least one spectra, said one-dimensional array functioning as the slit of said spectrometer.

5. The system of

claim 4

wherein said spectrometer is at least one of a dispersive spectrometer, a FT spectrometer, and a transmissive holographic spectrometer.

6. The system of

claim 4

further comprising at least one of a laser and illumination rejection filter which removes laser light from the perpendicular light.

7. The system of

claim 6

wherein said at least one of a laser and illumination rejection filter is at least one of a dielectric interference filter, a holographic optical filter or a rugate optical filter.

8. The system of

claim 7

wherein said dielectric interference filter is one of a notch bandreject and longpass type.

9. The system of

claim 1

wherein said light from said sample is at least one of Raman, fluorescence, photoluminescence, UV/visible absorption/reflectance, near infrared absorption/reflectance and Mid-infrared absorption/reflectance.

10. The system of

claim 1

wherein the spectra generating system output is a direct beam coupled to a spectrometer.

11. The system of

claim 1

wherein said system is incorporated into a microscope-based spectroscopic imaging system.

12. The system of

claim 1

wherein said system is incorporated into a macroscope-based spectroscopic imaging system.

13. The system of

claim 1

wherein said system is incorporated into a fiberscope-based spectroscopic imaging system.

14. The system of

claim 1

wherein said system is incorporated into a telescope-based spectroscopic imaging system.

15. The system of

claim 1

wherein said polarization beamsplitter is a polarizing cube beamsplitter.

16. The system of

claim 1

wherein said polarization beamsplitter is a polarizing thin film beamsplitter.

17. The system of

claim 1

wherein said polarization beamsplitter is a laser line rejection filter acting as a beamsplitter having improved angular field of view, reduced sensitivity to angular adjustment and improved rejection efficiency for widefield imaging system.