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Apparatus and methods for parallel processing of micro-volume liquid reactions

  • US 20010055765A1
  • Filed: 02/20/2001
  • Published: 12/27/2001
  • Est. Priority Date: 02/18/2000
  • Status: Abandoned Application
First Claim
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1. A method for simultaneously conducting a plurality of micro-volume reactions, the method comprising:

  • (a) introducing a plurality of liquid samples into the sample chambers of a microhole apparatus, wherein the samples contain necessary reaction components; and

    (b) placing the apparatus into an environment favorable to the reaction;

    wherein the microhole apparatus comprises a substrate, wherein the substrate defines a plurality of sample chambers, wherein each sample chamber;

    (i) extends through the substrate;

    (ii) comprises one or more walls and an opening at each end; and

    holds a sample such that the sample is in the form of a thin film such that a liquid sample present in one sample chamber does not intermix with a liquid sample present in another sample chamber; and

    wherein the sample chamber has a height to width ratio of less than 1;

    1, wherein the height of the sample chamber is measured from one face of the substrate to the other.

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